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37 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Treponema pallidum
Disease? |
Syphilis
Pathogen? Shape? |
T. pallidum Spirochete |
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T. Pallidum
Shape |
Spirochete T. pallidum
Disease? |
Syphilis |
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T. Pallidum
How many types of test there are? |
2
List |
Screening test Confirmatory tests |
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T. Pallidum
2 types of test |
Screening test Confirmatory test |
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T. Pallidum
_____________ Confirmatory test |
Screening test
Test name |
RPR/VDRL test |
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T. pallidum
Screening test
How many there are? |
1
List |
RPR/VDRL test |
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T. pallidum
1 screening test
List |
RPR/VDRL test
Abs against? |
Cardiolipin |
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T. pallidum 1 screening test: RPR/VDRL test
Abs against? |
Cardiolipin
Test name Range? Peak? |
T. pallidum
Range: + in 1-4 weeks after primary stage to latency
Peak in secondary or beginning of latent stage |
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T. pallidum
Screening test: RPR/VDRL Range? Peak? |
Positive in 1-4 weeks after primary stage to latency
Peak at Secondary or beginning of latent stage |
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T. pallidum
Screening test: RPR/VDRL
Procedure
How many steps? |
3
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Pt. serum + Cardiolipin-cholesterol-lecithin-charcoal complex
Mixed or rotated
Observation |
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T. pallidum Screening test: RPR/VDRL
3 steps |
1. Pt. serum + cardiolipin+cholesterol+Lecithin+charcoal complex
2. Mix/rotate
3. _________+ interpretation |
Observation
Flocculation: + anti-cardiolipin
No floculation: (-) anti-cardiolipin |
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T. pallidum
Screening test: RPR?VDRL
Observation and interpretation of results? |
Flocculation: + anti-cardiolipin No flocculation: - anti-cardiolipin
Positive results must be confirmed by? |
Confirmatory test |
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Confirmatory test are also known as? |
Treponemal test |
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Why confirmatory test are needed to confirm T. pallidum? |
Specific: Abs are against T. pallidum
Becomes positive early in infection
Reactive for life |
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Why confirmatory test are needed to confirm T. pallidum?
How many reasons? |
3
List |
Specific: Abs are against T. pallidum
Becomes positive in infection
Reactive for life |
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Review: Timing of confirmatory test of T. pallidum
Range |
Early
Reactive for life
Review: Screening (RPR or VDRL) |
Positive in 1-3 weeks after primary infection to latent infection
Peaks at secondary or latent |
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Confirmatory test
Test name? |
FTA-ABS MHA-TP |
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Confimatory test
How many? |
2
List |
FTA-ABS MHA-TP |
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Confimatory test
____________ MHA-TP |
FTA-ABS
Procedure |
1. T. pallidum immobolized on microscope slide + Absorbed Pt. serum
Incubate/Wash
2. Anti-Human Ig-FITC
Incubate/wash
3. Observation with fluorescent microscope |
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Confimatory test FTA-ABS
Procedure
How many steps? |
3
List |
1. Pt. serum + T. pallidum immobolized on microscope slide
Incubate and wash 2. Anti human-Ig FITC
Incubate and wash
3. Observation with fluorescent microscope |
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Confimatory test
FTA-ABS
Procedure
3 steps |
1. Pt. serum + T. pallidum immobolized on microscope slide
I and W
2. Anti human Ig-FITC
I and W
3. ______________+ interpretation |
Observation with F microscope
Fluorescence: + Ab
No Florescence: - Ab |
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FTA-ABS
Interpretation of results |
Fluorescence= No fluorescence= |
+ - Ab |
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Borrelia burgdorferi
Disease? |
Lyme disease
Pathogen: B. burgdoreferi
Shape |
Spirochete |
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B. burgdoreferi
Disease? |
Lyme disease
Notes |
Found in white-footed mouse, tick vector |
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Lyme disease
Production of Abs (Time ) |
3-6 weeks |
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Lyme disease
Two types of test? |
Screening Confirmatory tests |
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Lyme disease
____________ Confirmatory tests |
Screening test
How many? |
2 |
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Lyme disease
Screening test
How many? |
2
List |
IFA EIA |
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Lyme disease
2 screening tests |
IFA EIA |
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Lyme disease
Positive results are confirmed by? |
Western blot
Procedure How many steps?
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4 |
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Lyme disease
Positive results are confirmed by western blot
4 steps
List |
1. Electrophoresis of B. Burgdorferi ag and then blooted onto nitorcellulose paper
2. Incubated on paper with primary Ab
3. Incubated on paper with secondary Ab
4. add the enzyme substrate |
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Lyme disease
Western blot
4 steps |
1. Electrophoresis of B. burgdoferi antigens Ag and then blotted on nitrocellulose paper
2. Incubate on paper with primary Ab
3. ________________+name of that Ab?
4. Add enzyme substrate |
Incubate on paper with secondary Ab
Enzyme-labeled anti-human Ig |
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Lyme disease
Western blot
4 steps 1. Electrophoresis of B. Burgdoferi Ags and then blotted on nitrocellulose paper
2. Incubated on paper with primary Ab
3. Incuabted on paper with secondary Ab + description
4. _______________ |
Seconary Ab: enzyme labeled anti-human Ig
Add enzyme substrate
Purpose? |
Visualization |
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Lyme disease
Western blot
Interpretation of results |
Band visible= Band not visible= |
+ for that Ab - for that Ab |
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Lyme disease
Western blot
Band visible Band not visible |
_____________= + for that Ab _____________= - for that Ab |
Band visible Band not visible |
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Lyme disease
If IgM used, what is the characteristic molecular weight for a positive result? |
2 of 3 bands
What about IgG? |
5 of 10 bands |
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Lyme disease
If IgG used, what is the characteristic molecular weight for a positive result? |
5 of 10 bands
What about IgM? |
2 of 3 bands |
