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23 Cards in this Set
- Front
- Back
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What type of antigen do T-cells see?
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ONLY see peptide antigen
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TCR binds?
CD molecule binds? |
TCR = peptide + MHC
CD (4 or 8) = MHC |
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principle fxn of dendritic cells?
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Professional antigen presenting cell: principal function is to initiate T cell responses and bring antigen from site of infection to lymph nodes
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Dendritic cells present antigen to
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NAIVE T-cells
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Conventional Dendritic cells:
Surface markers = Major Location = Expression of TLR = Major cytokines produced = Major function = |
Surface markers = High CD11
Major Location = Tissues Expression of TLR = 4, 5, 8 Major cytokines produced = TNF, IL-6, IL-12 Major function = induction of T-cells |
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Plasmacytoid Dendritic cells:
Surface markers = Major Location = Expression of TLR = Major cytokines produced = Major function = |
Surface markers = CD 11 low, B220 high
Major Location = blood AND tissue Expression of TLR = 7, 9 Major cytokines produced = Type-1-IFN Major function = antiviral innate immunity and induction of T-cells |
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Cells that produce MHC-1? MHC-2
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MHC-1 = all NUCLEATED cells
MHC-2 = b-cells, macrophages, APCs, and epithelial cells of the thymus |
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Where is the MHC gene loci?
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chromosome 6
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MHC-1:
Gene loci = Chain make up = presents antigen from = |
MHC-1:
Gene loci = HLA-A, HLA-B, HLA-C Chain make up = alpha-chain + B2 microglobulin presents antigen from = ENDOgenously derived sources |
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Is B2 microglobulin part of the HLA locus?
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No, it is non-polymorphic and outside the HLA locus
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For MHC class I, the cleft is formed by the folding of ?
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the first two domains (out of 3) of the class I molecule aka alpha-1 and alpha-2
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MHC-2:
Gene loci = Chain make up = presents antigen from = |
MHC-2:
Gene loci = HLA-DR, HLA-DP & HLA-DQ Chain make up = alpha chain and beta chain from HLA locus presents antigen from = exogenously-derived/extracellular peptide antigens |
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MHC2:
What type of bond is between the alpha chain and beta chain in the cleft? Is the beta chain polymorphic? |
NON-covalent
beta chain = polymorphic |
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The cleft in MHC class II is formed by
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the first domain (out of 3) of the α chain plus the first domain (out of 3) of the β chain
aka alpha-1 plus beta-1 |
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How many possible MHC-1 heterodimers?
How many possible MHC-2 heterodimers? |
MHC-1 = 6 possible
MHC-2 = 12 possible (Beta chain polymorphism) |
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The entire set of MHC alleles on a chromosome is called ?
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a haplotype - we inherit one haplotype from each parent
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In MHC-1 processing, after degradation by proteasomes, peptides are then transported across the _______ by a peptide transporter known as _______
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peptides are then transported across the endoplasmic reticulum (ER) membrane by a peptide transporter known as TAP (transporter associated with antigen processing)
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MHC class II+Invariant chain (Ii) complexes are transported to a specialized endosomal intracellular vesicle known as the ____________ where Ii is attacked by proteases, leaving a peptide from Ii known as _______ associated with the peptide binding cleft.
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MHC class II compartment (MIIC or CIIV)
CLIP |
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Subsequently, a non-polymorphic MHC protein known as _______ catalyzes the release of _______ from MHC class II thereby allowing peptides derived from endocytosed extracellular proteins to gain access to the nascent MHC class II molecules and be transported to the cell surface.
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HLA-DM catalyzes the release of CLIP
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Function of invariant chain?
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Li = invatiant chain = binds to MHC2 to stabalize it from binding normal intracellular proteins that are in the ER.
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Is HLA-DM a class 2 molecule?
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no
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antigen presentation by macrophages to CD4 T-cells leads to
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cytokine release that further activates macrophages
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antigen presentation by B-cells to CD4 T-cells leads to
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cytokine release that leads to antibody release
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