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36 Cards in this Set
- Front
- Back
Name the two places extracellular infections occur and how they contribute to protective immunity.
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1. Interstitial spaces, blood, lymph: antibodies, complement, phagocytosis
2. Epithelial spaces: igA antibodies, Antimicrobial peptides |
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Name the two places intracellular infections occur and how they contribute to protective immunity.
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1. Cytoplasm: cytotoxic T cells, NK cells
2. Vesicular: T-Cell, NK-cell activated macs |
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What are the three distinct phases of the infectious process?
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1. Innate Immunity: 0-4hrs
2. Non-adaptive, early induced immunity: 4-96hrs 3. Adaptive immunity >96hrs |
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Name 4 of the barriers/cells involved in the Innate immunity response [0-4hrs]?
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1. Epithelial barrier
2. Local chemical factors 3. Alternate complement pathway 4. Pre-formed, nonspecfic effector cells: Phagocytes [neutrophils, macs] |
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Explain how neutrophils bind many bacterial constituents
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Surface receptors ex] mannose receptor, LPS, CR4, glycan, scavenger, CR3
-Neutrophils digest pathogens that cross-link these receptors |
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What vesicular products are of functional significance in the innate immune response?
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1. Acids (drop vesicular pH to 3.5-4)
2. toxic oxygen derivatives (O2-, H2O2, OH radical, oxygen radical, Ocl-hypohalite, NO) 3. Peptides/proteins (defensins, cationic proteins) 4. Enzymes (myeloperoxidase, lysozyme, acid hydrolase) 5. Competitors (lactoferrin, B12 binding protein) |
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What are Toll-like receptors [TLR's] and what stage of immunity are they involved in?
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they are found on cells likely to encounter pathogens first [like epithelial cells and phagocytic cells]
their receptors recognize different classes of pathogens [ie. viruses, bacteria, pathogens]. They often use a common pathway of signal transduction Involved in non-adaptive, early immunity [4-96hrs] |
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Name the mediators/cytokines that phagocytes release to induce an inflammatory response
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IL-1
IL-8 TNF-alpha IL-6 IL-12 CXCL8 |
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What are the local and systemic effects of IL-1?
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LOCAL: activate vascular epithelium and lymphocytes
Local tissue destruction Increase access to effector cells SYSTEMIC: fever and production IL-6 -->also activates ACUTE PHASE RESPONSE |
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What are the local and systemic effects of TNF-alpha? **
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LOCAL:
-activate vascular epithelium -increase vascular permeability -increase IgG entry-->complement -increase fluid drainage to lymph nodes SYSTEMIC: -fever -mobilization of metabolites -SHOCK -->can also activate ACUTE PHASE RESPONSE |
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What are the local and systemic effects of IL-6?
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LOCAL:
-lymphocyte activation -increase Ab production SYSTEMIC: -fever -->can also activate ACUTE PHASE RESPONSE |
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What are the local effects of IL-8, IL-12, and CXCL8?
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IL-8:
LOCAL: -chemotactic factor for leukocytes (attracts them to the infection) -increase access of effector cells -activates binding of Beta 2 integrins --activation of PMNs--neutrophils, eosinophils, basophils--(along w/TNF-a) IL-12: LOCAL: -Activate NK cells -induce differentiation of CD4 T cells-->TH1 cells CXCL8: -chemotactic factor for neutrophils, basophils, and T cells (attracts them to infection)--BIG role in diapedesis |
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What is diapedesis and what causes it?
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the migration of leukocytes from blood vessels
Caused by inflammatory mediators that activate adhesive reactions [endotoxins]--CXCL8 plays big role -->there are a zillion types of chemicals used to attract molecules to site in inf--did he say we need to know these? fig 8.16, 8.19 |
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Explain how SHOCK occurs in the systemic infection
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Macs activated in the liver/spleen secrete TNF-alpha into BLOOD (instead of tissue)
Causes: -systemic edema -hypotension [leading to a collapse in vessels] -neutropenia followed by neutrophilia -Disseminate intravascular coagulation [DIC] leads to organ wasting and multiple organ failure Result: death |
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What are CHEMOKINES?
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Small, chemoattractant polypeptides that amplify immune response by recruiting more phag. and immunocompetent cells to the site of infection
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What induces the ACUTE PHASE RESPONSE?
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cytokines IL-1, IL-6, TNF-aplha
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What are two of the acute phase proteins and what is their function?
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1. C REACTIVE PROTEIN: bind to phsophorylcholine on bacterial surfaces, acting as an opsonin and activate complement cascade by binding to C1q
2. MANNAN-BINDING LECTIN: bind to mannose on bacteria and acts as oposin activating complement by the lectin activation pathway [via C4 and C2] |
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8 important things occur in the non-adaptive, early induced immunity. What are they?
....you can do it! |
1. Phagocytes release cytokines-->induce inflammatory response
2. Diapedesis of leukocytes from blood vessels-->infection 3. Release of TNF-alpha [double-edged sword b/c locally protective, systemically shock] 4. Release of chemokines-->rectruits more phagocytes to infection 5. Induction of acute phase response (IL-1, 6, TNF-alpha) 6. Production of interferon-alpha and interferon-beta [by virus infected cells] 7. Induction of natural killer cells 8. Induction of gamma, delta cell T cells-->important in early infection |
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What cells are the major lymphoid population in epitheloid tissues and muscosa but only represent 1-5% of T cells in peripheral lymphoid organs?
Note: They are are restricted by MHC Class I molecules like CD-1... |
Gamma, delta T cells
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Who are the main players for adaptive immunity and when does it occur and why does it take so long to kick in?
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B lymph and T lymph
>96hrs clonal expansion! |
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What is the result of adaptive immunity?
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long lasting protection through induction of memory lymphocytes
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Who are the key players in humoral/adaptive mediated immunity?
where does it occur? what cytokine induces this response? |
Ag. specific B cells and CD4 helper T cells TH2 type [and APC]
extracellular induced by IL-4! |
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Who are the key players in cell mediated immunity
where does it occur? what cytokines induce it? |
CD4 inflammatory T cell [TH1]--releases IL-2, activating CD8 cytotoxic T cells
-intracellular -induced by IL-12 and IFN gamma! IL-12 activates NK cells-->NK cells make IFN-gamma-->activates TH1-->releases IL-2-->activates CD8! |
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How are Tuberculoid leprosy and Lepromatous leprosy different?
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Tuberculoid leprosy: well controlled by TH1 cells that activate infected macs. Inflammation localized [dominant cytokines--IL2, interferon-gamma,TNF Beta]
Lepromatous leprosy: uncontrolled TH2 cells, thus leprosy widely disseminated and severe damage caused [dominant cytokines: IL4, 5, 10] |
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What are cytokines released by each type of T-cell?
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TH1 (INFLAMMATORY)
-IL-2 (activates T CELL GROWTH-->including CD 8!) -IL-3 (hematopoeisis--makes more LEUKOCYTES) -IFN-GAMMA (enhances INFLAMMATION, activates phagocytes) -TNF-ALPHA (enhances INFLAMMATION, increases vacular PERMEABILITY) TH17 (INFLAMMATORY) -IL-17 (Chemokines for NEUTROPHIL recruitment, activates IL-6, acute phase proteins, works w/IFN-GAMMA, TNF-ALPHA) TH2 (Helper) -IL-3 (hematopoeisis--makes more LEUKOCYTES) -IL 4 (B cell ACTIVATION, IgM-->IgE isotype switch, SUPPRESSES TH1, TH17) -IL-5 (B cell/eosinophil GROWTH) -IL-10 (suppresses TH1 cytokines) -TGF-BETA (suppresses TH1 cytokines) |
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What ig shows up first during a primary infection [amount of ab and highest affinity]?
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IgM
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Which ig has the highest antibody amount and affinity in the secondary and tertiary infection?
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IgG [due to isotype switching]
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Which cell appears from 0-96hrs and is the 1st line defense against infection [bacteria, fungi, parasites]?
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Neutrophil
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What cell appears from 0-96hrs and also the first line of defense, but is involved in antigen presentation and is the link to adaptive immune response in pathogens?
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macrophages
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What cell is involved from 4-96hrs and is the first line of defense against VIRUSES
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Natural Killer
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A virus titer shows:
which cytokines are released in a burst as a result of infection? These cytokines induce proliferation of what? At the end of the titer [5 -10days], what cells are developing? |
IFN-gamma, TNK-alpha, IL-12
induce NK killing of infected cells [control virus replication and spread of infection] effector CD8 T cells begin to develop after 5days |
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What are the two mechanisms that extracellular bacteria cause disease?
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1. induce INFLAMMATION to destroy host tissue at the site of infection
2. produce TOXINS which have diverse pathological effects |
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How do ENDOTOXINS differ from EXOTOXINS?
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ENDOTOXINS-a bacterial toxin found naturally inside pathogen. Not secreted but rather a structural component which is released when the bacteria is lysed
: ie bacterial cells wall like LPS EXOTOXINS- soluble protein secreted by microorganisms. it is formed within the cell, but is released into the environment where it is rapidly active in extremely small amounts ie] cholera toxin, teatnus toxin |
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What are the cytokines released by phagocytes during the non-adaptive, early induced immunity?
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Phagocytes release:
IL-1, 6, 8, 12, TNF-alpha, CXCL8 Help induce inflammatory response and amplifies immune response. |
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What is difference between cytokines and chemokines?
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cytokines--proteins made by cells that affect behavior of other cells. Can be released by lymphocytes or phagocytes.
chemokines--proteins released that guide WBC's to infection |
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What factors determine whether an infection uses humoral vs. cell-mediated immunity?
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HUMORAL
--Induction of IL-4-->TH2 response! CELL-MEDIATED --Induction of dendritic cells-->IL-12-->activates NK cells-->NK cells make IFN-gamma-->TH1-->makes IL-2-->activates CD8 cells! |