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6 Cards in this Set

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What are the targets, primary reag, secondary reag and detection system used for ELISA assay?
ELISA
Target: proteins, sugars, lipids, cells, cell lysates
Primary: standard Ab to target;unknown
Secondary: Ab against Primary; Fc coupled to enzyme
Detection: chromagen; color rxn
What are the targets, primary reag, secondary reag and detection system used for Immunoblot?
Target: proteins
Everything else same as Elisa but performed on a sheet containing transfered Ag
What are the targets, primary reag, secondary reag and detection system used for Immunohistochemistry?
An ELISA perfomed on cells or tissues; visual scoring
What are the targets, primary reag, secondary reag and detection system used for Immunofluorescence?
Target: cells, tissue
Primary:anti-target Ab
Secondary: Ab against Primary; Fc coupled to fluors (FITC, RITC)
Detection: fluorescing complex uv activated;microscope or FACs
What are the targets, primary reag, secondary reag and detection system used for Immunolocalization (EM)?
Target:proteins, sugars,lipids,cells, tissue
Primary: Anti-target Mab
Secondary:Ab against primary; Fc coupled to gold particles of defined size
Detection: electron microscopy; visual
What are the targets, primary reag, secondary reag and detection system used for Radioimmunoassay (RIA)?
Target: proteins, sugars, lipis, cells, cell lysates
Primary: Anti target Ab
Secondary:Ab against Primary: Fc labeled with isotope
Detection: determination of cpm bound