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6 Cards in this Set
- Front
- Back
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What are the targets, primary reag, secondary reag and detection system used for ELISA assay?
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ELISA
Target: proteins, sugars, lipids, cells, cell lysates Primary: standard Ab to target;unknown Secondary: Ab against Primary; Fc coupled to enzyme Detection: chromagen; color rxn |
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What are the targets, primary reag, secondary reag and detection system used for Immunoblot?
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Target: proteins
Everything else same as Elisa but performed on a sheet containing transfered Ag |
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What are the targets, primary reag, secondary reag and detection system used for Immunohistochemistry?
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An ELISA perfomed on cells or tissues; visual scoring
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What are the targets, primary reag, secondary reag and detection system used for Immunofluorescence?
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Target: cells, tissue
Primary:anti-target Ab Secondary: Ab against Primary; Fc coupled to fluors (FITC, RITC) Detection: fluorescing complex uv activated;microscope or FACs |
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What are the targets, primary reag, secondary reag and detection system used for Immunolocalization (EM)?
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Target:proteins, sugars,lipids,cells, tissue
Primary: Anti-target Mab Secondary:Ab against primary; Fc coupled to gold particles of defined size Detection: electron microscopy; visual |
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What are the targets, primary reag, secondary reag and detection system used for Radioimmunoassay (RIA)?
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Target: proteins, sugars, lipis, cells, cell lysates
Primary: Anti target Ab Secondary:Ab against Primary: Fc labeled with isotope Detection: determination of cpm bound |
