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42 Cards in this Set

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Steps in Viral replication
attachment
entry & uncoating
replication
assembly
release
attachment of HIV
binds CD4 & co-receptor of T cell
attachment of EBV
binds CD3 (complement) receptor on B cell
attachment of Rabies
binds ACh R on neuron
attachment of Influenza A
binds sialic acid on epithelial cells
hemagluttination
Attachement of viruses
random, electrostatic, viral ligand/host R, independent of temp
Entry & Uncoating of Virus
entire virus or nucleocapsid into cytoplasm, then remove capsid

RM endocytosis (both)
Direct penetration (naked)
Fusion (enveloped)
receptor mediated endocytosis
for either enveloped or naked
requires low pH to releas from vesicle
direct penetration
entry for non-enveloped virus (no vesicle)
fusion
entry for enveloped virus (no vesicle)
Methylisoxazole
interferes with uncoating of Picornavirus
Amantidine
(& Rimantidine)
interfere with uncoating of influenza
Tromantadine
inhibits penetration of herpes
Enfuvirtide
inhibits fusion of HIV w/CD4
Viral Replication DNA viruses
Early- virion DNA
Late- Progeny
Translation depends on capping, splice sites, polyadenylation
End Problem
Early replication
part of DNA virus replication
make proteins needed to subvert host machinery
requires DNA dependent RNA polymerase
Late replication
part of DNA virus replication
make progeny
requires DNA polymerase
ss virus must synthesize a ds intermediate
End Problem
in DNA virus replication
Polyoma & Papilloma are circular- no problem
Adeno- linear, synthesizes primer proteins
HBV uses reverse transcriptase
RNA dependent RNA polymerase
carried by all RNA viruses
for replication
(retrovius carries RNA dependent DNA polymerase)
replication of ss +RNA virus
ss +RNA reads like mRNA
may make ds intermediate
DNA intermediate can integrate into host
replication of ss -RNA virus
RNA dependent RNA polymerase to synthesize mRNA from - strand
replication of segmented ss -RNA virus
happens in NUCLEUS
Cap snatching to initiate mRNA synthesis
Cap Snatching
segmented ss -RNA virus uses to initiate mRNA synthesis
Capped RNA primers are cleaved from cellular nuclear RNA
replication of ds RNA
synthesize + strand from - strand of parent
replication of retrovirus
use - strand DNA intermediate to make + strand RNA progeny
Quasi species
found in RNA virus
advantage over host b/c always changing
high mutation rate
rare direct viral damage
fever, HA, rash
Shutoff
Poliovirus
Shuts off host cell machinery
cell dies after virus leaves
virus cleaves complex responsible for cap dependent translation
outcome of non-HIV retro viral infection
persistant infection
outcome of HIV, Hep B & C infection
chronic infection
latent infection caused by
lambda virus in E coli
beta virus in diphtheria
HSV
cellular changes due to viral infection
Inclusion bodies- Herpes
Syncytia- Measles
Transformation- leads to tumor
Apoptosis & Cellular Degeneration
Ebola Virus Toxin
Glycoprotein
Adenovirus Toxin
penton
Rotavirus toxin
NSP4
Viral Toxins
Ebola- glycoprotein
Adeno- penton
Rota- NSP4
Viral Escape Mechanisms
IFN activates genes in some virus
Escape N ABs & CTLs
ACE-2
receptor for SARS
delta ORF
virulent SARS
SARS
coronaviridae
ACE-2 is R
delta ORF was mutation
never efficient enough to switch to human host
reservoir is bat
Influenza
8 segments in genome
surface GPs (HA & NA)
HA is fusion prot
NA cleaves sialic acid
protein N1 inhibits IFN activation
Vector Borne Illnesses
from Flavivirus
Grippe-like (Dengue) Fever-
fever, bone pain, loss ap
DHF/DSS- shock, cap leak
West Nile, SLE: flu, enceph
Yellow Fever: hem fever, cap
leak, hepatitis