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28 Cards in this Set
- Front
- Back
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Sample Prep:
Eco 0157 |
375g + GN @15hrs |
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Sample Prep: Salmonella |
375g + GN @ 15hrs |
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Sample Prep:
EHEC |
375g + GN @15hrs |
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Sample Prep:
Lg/ Lm Listeria |
375g + GP @ 21hrs |
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Sample Prep: STAK |
375g + GN @15hrs |
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Sample Prep: WPA samples, incubation times for GN and GP |
15hrs for GN, 21hrs for GP |
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Sample Prep: Flour |
375g of sample + 2 bottles of media @1:5 dilution |
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Sample Prep: Carrot fiber |
25g of sample + 2bottles of media @1:50 dilution |
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Sample Prep: Fresh produce (garlic, lettuce, cabbage, ect.) |
375g sample + 1bottle @1:3 dilution for 9Hrs |
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What media is used for TCC/ECC in MPN test? |
LTB media, done in triplicates of dilutions 6,7,8 |
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For MPN test, What media is used to confirm E.coli? |
ECMug, incubated over 24 hours. positive is confirmed by: Turbidity Gas UV fluoresence |
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For MPN test, what media is used to confirm Coliforms? |
BGB, incubated over 24 hours. positive is confirmed by : turbidity Gas color change to yellow |
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Sample Prep: full micro, or any micro order |
25 grams of sample + 225g of BPW for 1:10 dilution |
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+ control for VRBG X-Gluc |
E. coli |
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+ control for VRBG |
Klebsiella aerogenes (KA) formerly known as Enterobacter aerogenes |
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Klebsiella aerogenes |
Gram Negative, motile (peritrichous), Facultative anaerobe |
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What do DRBC plates test for? |
Yeast and Mold |
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For how long and where/what temperature are DRBC plates stored |
5 days @ 27C stored in incubator 21 |
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Outline benchtop protocol |
1) soak 7 areas in alcohol and bleach 2) Make environmental SARF form 3) Make sticker labels, three copies 4) Collect sponges, and go to each station and dry, the swab area 5) label sponges, 7 bags, and 7 AC petrifilms 6) plate 1mL of sponge to AC mf INC13@48hrs 7) add 20mL of PCT GN squeeze, move 15mL to new bag 8) new bag + 50mL GN 21hrs INC17 9) SP bag + 50mL GP 21hrs INC 5 |
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in Benchtop, Sponge bag gets what media? |
GP media |
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in Benchtop, new bag gets what media? |
GN media |
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how long are benchtop sponges incubated for? |
21 Hours |
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WPA salmonella Sponges Sample prep |
Add {[#sp/Bag] * 90mL GN media} incubate in INC15 @ 15hrs |
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Salmonella sponges sample prep |
Add {[#sp/Bag] * 90mL GN media} incubate in INC15 @ 12hrs |
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In Y/M microfilm, mold looks like |
DIFFUSE BLUE COLONIES |
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In Y/M microfilm, YEAST looks like |
defined edge blue colonies |
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Sponge E.coli test sample prep |
Add 90mL of BPW Massage 30X Plate on EC petrifilm -1 dilution (24hrs INC13) |
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Ready Roast E.coli micro test for Finished product |
X- Gluc spread plate, duplicates -1, -3 |
