Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
22 Cards in this Set
- Front
- Back
|
(12)Name Of Test
Hydrolysis Of Gelatin |
Medium-Nutrient gelatin
Substrate-gelatin protein polymer Enyzme/path-gelatinose/hydrolysis ph ind/Reagents-zero End product- tre Amino Acids Positive- +Liquids-P.vulgans +,B.subtilis appearance. Negative Appearance -Solid line of bacteria (-) ve |
|
|
(13)Name Of Test
Fermentation Of Carbohydrates |
Medium- Sugar Broth
Substrate-CHO e.g SUCROSE Enzyme/path - Phenol Red End Product - ATP & Organic Acid & ATP Organice Acid & Co2 Pos Appearance- Yellow (A) (+) Yello w + gas bubble + Neg - Red (-) (B) No fermentation Pink -(-) Alkligense |
|
|
(14)Name Of Test
Fermentation (Clarity) |
Medium- (Clarity) Litmus Milk
Substrate - Lactose Enzyme/path - Fermination Ph.Ind/Reagents - zero End Product - ATP & Organic Acid & Hard Curd(protein) & Co2. Pos. (+) Appearance- Pink & Hard Curd (solid) & Gas (swiss Cheese) Neg (-) - Purple -Control-Blueberry,Grapejuice |
|
|
(14)Name Of Test
Alkalinization(Carbon) |
Litmus(Carbon) Milk
Substrate - Lactose Enzyme/Path -Alkalinization -Partial Hydrolysis Ph.Ind/Reagents - Zero End Prod- Polypeptide(colloid) Basic Amino Acids ( ) Pos(-) -Blue Milk ( ) Neg(-) - Purple -Control Blueberry,Pink,Grape juice |
|
|
(14)Name Of Test
Peptonization- (Cloudy) |
Medium - Casein & Lactoablbumin
Enzyme/Path - :Peptonization :Comple Hydrolysis(by accepting e -) Ph Ind./Reagents -Amino Acids (A.A) (Crystalloid) LESS TIRBID Pos (+) Appear Juice- Dark Liquid Neg(-) Appear Purple- Control Blueberry Pink |
|
|
(14)Name Of Test
Reduction (Curd) |
Medium -Litmus(Curd)
Substrate - Proteins + ? Enzyme/Path - ---------- pH Ind./Reagents - Zero End Prod -- Amino Acids(A.A) & Rennet & Curd -( COTTAGE CHEESE) Pos(+) Appear - Transluent + Soft And i Bowel Full i Neg - AppearPurple-Control Blueberry, Pink |
|
|
(14)Name Of Test
Curd Formation |
Medium - Litmus Milk
Substrate -Litmus Enzyme/Path - Reduction ETC :Anaerobic Resp pH Ind/Reagents - Zero End Product - Leucolitnus (Reduced litmus) & ATP Pos Appear (+) Bleached litmus[no Pink,Purple Blue] Neg Appear (-) litmus Colors Purple,Blue,Pinkl |
|
|
(15)Name Of Test
Reduction Of Nitrates |
Medium -Nitrate Broth
Substrate - Nitrate Enzyme /Path - AnaerobicRespiration pH.Ind./Reagents - 3gtts Of Sulfonilic Acid,2Gtts Of Dan Solution. End Prod: - ATP & No2(Nitrate) Pos Appear: -Red(+) / Magenta(+),B.subtilis. Neg.Appear: - Any Other Color A.faecalis (-) Control (-) |
|
|
(16)Name Of Test
Detection Of Catalase |
Medium - tryptic soy agar
Substrate - Hydrogen Peroxide Enzyme/Path:- Aerobic Catalase pH Ind./Hydrogen Peroxide,H2o,o2 End Product - H2o + o2 (water + oxygen) Pos Appear:- Bubbles (+) Neg Appear: - No Bubbles (-) |
|
|
(17)Name Of Test
Production Of Decarboxtylase |
Medium - Amino Acid Broth
Substrate: Amino Acid e.g- Lsine Enyzme /Path - Decarboxylation Decarboxylation pH Ind./Reagents Brom -Cresol-Purple [ {}] End Prod: Basic Amine & Increase Co2 Pos Appear: (+) Violet (+) Neg Appear: (-) Burgundy & Brown(-) |
|
|
(18)Name Of Test
Hydolysis Of Urea |
Medium:Urea Broth
Substrate-Urea Enyzme/Path: Urease pH Ind./Reagents:-Phenol Reds End Prod: -Co2 Increase & Basic Ammonia(NH3) Pos.Appear: - Hot Pink/Magenta( +) Neg.(-)Appear: -Yellow ( -) [only milliter] |
|
|
(19)Name Of Test-3tubes in 1
Production Of Hydrogen sulfric gas (H2s) |
Medium -(Tall) SIM
Substrate: - Cysteine (s) (A.A) Enzyme/Pathway- Anaerobic respiration ph Ind./Reagents: -Zero End Prod: -ATP & H2 s Decrease,Black Iron Sulfide Fe++ Agar Pos.(+)Appear: - H2s + Fe 2+ [ ] Black (+) Neg(-).Appear: Not Black |
|
|
(19)Name Of Test-3tubes in 1
Production Of Indole |
Medium: TALL-(tryptophan)
SIM Substrate: - tryptophan Enyzme/Path:-Indole Metobolism pH.Ind/Reagents:- Zero End Prod: - Indole Pos.Appear: RED Kovas's [[[ Neg Appear:(-)Not RED |
|
|
((19)Name Of Test-3tubes in 1
Motility |
Medium: -Tall-SIM
Substrate:- N/A Enzyme/Path: - N/A pH Ind./Reagents: - N/A End Products: - N/A Pos.(+) Appearance: - Turbidity(Cloudy) Neg (-)not Cloudy |
|
|
(20) Name Of Test
Methyl Red Test Acid pH(<4.4) |
Medium: -MR-VP
Broth Substrate: - Glucose Enyzme/Path: - Fermentation pH Ind./Reagents: - 1 ML Of Broth test tube add 10gtts of Methyl Red. End Prod: - Zero Pos(+) Appear: -RED(+)[Acid Present] Neg(-) YELLOW(no Acid) |
|
|
(20)Name Of Test
Voges-- Pwkaur Test |
Medium: - MR-VP
Broth Substrate: - Glucose Enzyme/Path: - Fermentation pH Ind./Reagents: - 1ML of brth into clean test tube,add 10gtts of K+ hydroxide,20-30 mins Creatine End Of Prod: - Zero Pos (+) Appear: - RED RING Neg(-) Appear: -Any Color(yellow,orange) |
|
|
(21)Name Of Test
Utilization Of Unusual Nitrogen |
Medium: - Ammonium
Phosphate Substrate: - Nitrogen Enyzme/Path: - Nitrogen Metabolism. pH Ind./Path: - Zero End Prod: - Ammonia(NH3) Phosphoric Acid-H3Po4) Pos.(+)appear: - YELLOW Neg.Appear:(-) NOT YELLOW |
|
|
(22)Name Of Test
Utilization Of Unusual Carbon |
Medium: - Sodium Citrate
Substrate: - Carbon Enyzme/Path: - :Citratase Carbon Metabolism pH.Ind/Reagents; - Growth End Products: - Zero Pos.(+) Appear:- Turbidity (+) Neg (-) Appear: (-) Clear |
|
|
(24)Name Of Test
MSA/STAPH |
Medium: - Mannitol SAlt Agar
Substrate: - Mannital Enzyme/Pathway:Fermentation pH Ind.?Reagents: - PHENOL RED End Product: - ATP &Organic Acid Pos(+)Appear: - YELLOW(+) Differential,Agar Turns Yellow(Fermented) Neg.(-)Appear: - NOT YELLOW AGAR |
|
|
(25)Name Of Test
Coagulase Production |
Medium: -Rabbit Plasma
Substrate: - Plasma(CItrated) Enyzme/Path: - Coagukase pH Ind./Reagents End Product: - Coagulated Plasma(clotted) Pos(+) Appear: - Solid Neg(-)Appear: Liquid |
|
|
(26)Name Of Test
Hemolysin Production |
Medium: -BLOOD(sheep Agar)
Substrate: - |
|
|
(27)Name Of Test[bunsen burner,loop]
STAPHYLOCOCCUS LATEX AGGLUTINATION Latex Agglutination |
Medium: -
Substrate: - Enyzme/Path: - pH Ind./Reagents: -(+)Control (-) Control End Prod: - Pos.Appear: - (+) Agglutination Neg(-)Appear: - |
