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29 Cards in this Set
- Front
- Back
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Primary importance of FROZEN SECTIONS
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RAPID DIAGNOSIS
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3Fs
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FATS/FORMALIN/FROZEN SECTIONS
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Carbohydrate fixation
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ALCOHOLIC FIXATIVES
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Protein fixation
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NEUTRAL BUFFERED FORMALDEHYDE OR FORMALDEHYDE VAPOR
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Glycogen fixation
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ALCOHOL-BASED such as Rossman’s fluid or cold absolute alcohol
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MERCURIC CHLORIDE
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fixative of choice for TISSUE PHOTOGRAPHY
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Zenker’s fluid
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LIVER, SPLEEN, CONNECTIVE TISSUE FIBERS and NUCLEI
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Zenker’s-formol (Helly’s)
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PITUITARY GLAND, BM, BLOOD-CONTAINING ORGANS SUCH AS SPLEEN AND LIVER
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Heidenhain’s susa solution
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TUMOR BIOPSIES ESPECIALLY SKIN fixative
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Regaud’s (Moller’s/Muller’s) fluid
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CHROMATIN, MITOTIC FIGURES, GOLGI BODIES, RBC and colloid-containing tissues
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Orth’s fluid
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study of early degenerative process and tissue necrosis, demonstrates rickettsia and other bacteria
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LEAD FIXATIVES
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ACID MUCOPOLYSACCHARIDES (fixative)
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BOUIN’S
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fixation of embryos and pituitary biopsies
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KIDNEY structures
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Bouin’s is NOT SUITABLE FOR FIXING _______, lipid and mucus
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17
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Glacial acetic acid solidifies at ___ C
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Carnoy’s fluid
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CHROMOSOMES, LYMPH GLAND AND URGENT BIOPSIES
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Newcomer’s fluid
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fixing of mucopolysaccharides and nuclear proteins
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NITRIC ACID
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most common and fastest decalcifying agent
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PERENYI’S FLUID
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decalcifies and softens tissues at the same time
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X-ray or radiological method
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most ideal, most sensitive method for determining the extent of decalcification
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EPON (PLASTIC MEDIUM)
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Embedding medium for electron microscopy
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REGRESSIVE STAINING
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Manual H and E staining progressive or regressive?
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45 to 50C, approximately 6-10C lower than the mp of wax
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temp of flotation water bath
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ORCEIN
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vegetable dye extracted from LICHENS
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IODINE
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probably the oldest of all stains
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JANUS GREEN
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demonstrating MITOCHONDRIA
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PAS, AZOCARMINE
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Stain for the basement membrane
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TOLUIDINE BLUE, CRESYL VIOLET ACETATE
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Stain for Helicobacter pylori
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1.518
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Mountant refractive index should be as close as possible to that of the glass slide which is ___
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