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30 Cards in this Set

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This stain is a combination of two dyes. The dyes are?
H & E.
Hemotoxylin and Eosin
This stains acidic components of cells and tissues such as RNA and DNA because it has basic radicals.
Hemotoxylin
Features that stain with hematoxylin are called ?
basophilic
This is an acidic dye that stains basic components red or pink.
Eosin
In an H & E, which dye is more specific?
Hemotoxylin is very specific, Eosin is not.
Why is so much of the cell light pink when you stain with H & E?
Eosin is not very specific.
Why does the nucleus stain blue in H&E?
Because of the heterochromatin which has ionized phosphate in it.
The extracellular matrix will exhibit acidophilia because of ?
ionized amino acid groups
Why are special stains required to view elastic fibers?
Elastic fibers generally do not like to stain with H&E.
What color are elastic fibers in an elastic stain?
dark blue or black
What is considered the CT stain?
Masson's Trichrome
This stain appears vaguely red white and blue.
Masson's Trichrome
Masson's Trichrome stain is used primarily to detect ?
Collagen
A Masson's Trichrome stain contains three dyes. What is each supposed to target?
Nuclei, cytoplasm, CT
In a Masson's Trichrome stain, the nuclei appear? The cytoplasm? The CT?
The nuclei=blue
Cytoplasm=bright red
CT=aqua blue/green
In this technique, the tissue is impregnated with a reducible silver salt.
Silver Stain
Tissues or cells that stain with a silver salt are referred to as ?
argyrophilic
Nervous tissue and reticular fibers are often viewed with what kind of stain?
Silver stain
PAS=?
Periodic Acid Schiff
PAS stains what?
Stains glycogen and various carbohydrate containing molecules that contain an aldehyde group.
Appear dark magenta.
The mucin produced by goblet cells as well as basement membranes stain magenta with what stain?
PAS
Why do basement membranes stain with PAS?
because of their proteoglycans
This technique is commonly used to look at the conditioni of the Bowman's capsule in the cortex of the kidney.
PAS
This stain is usually reserved for blood cells.
Wright's Stain
In this stain:
Nuclei are dark blue-purple
Erythrocytes are pink-red
Eosinophilic granules are bright red
Basophilic granules stain a deep blue-purple
Wright's Stain
In these stains, the tissue takes on a different color than the dye.
Metachromatic stains
Heparin granules in mast cells staining reddish purple using a toluidine blue stain is an example of what?
Metachromatic staining
What is the goal of immunocytochemistry?
The goal is to visualize some component (antigen) in a tissue section by means of an antibody.
Polyclonal Immunocytochemistry
Polyclonal antibodies are directed against several antigens.
Monoclonal Immunocytochemistry
antibodies are directed against one antigen.