Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
30 Cards in this Set
- Front
- Back
|
This stain is a combination of two dyes. The dyes are?
|
H & E.
Hemotoxylin and Eosin |
|
|
This stains acidic components of cells and tissues such as RNA and DNA because it has basic radicals.
|
Hemotoxylin
|
|
|
Features that stain with hematoxylin are called ?
|
basophilic
|
|
|
This is an acidic dye that stains basic components red or pink.
|
Eosin
|
|
|
In an H & E, which dye is more specific?
|
Hemotoxylin is very specific, Eosin is not.
|
|
|
Why is so much of the cell light pink when you stain with H & E?
|
Eosin is not very specific.
|
|
|
Why does the nucleus stain blue in H&E?
|
Because of the heterochromatin which has ionized phosphate in it.
|
|
|
The extracellular matrix will exhibit acidophilia because of ?
|
ionized amino acid groups
|
|
|
Why are special stains required to view elastic fibers?
|
Elastic fibers generally do not like to stain with H&E.
|
|
|
What color are elastic fibers in an elastic stain?
|
dark blue or black
|
|
|
What is considered the CT stain?
|
Masson's Trichrome
|
|
|
This stain appears vaguely red white and blue.
|
Masson's Trichrome
|
|
|
Masson's Trichrome stain is used primarily to detect ?
|
Collagen
|
|
|
A Masson's Trichrome stain contains three dyes. What is each supposed to target?
|
Nuclei, cytoplasm, CT
|
|
|
In a Masson's Trichrome stain, the nuclei appear? The cytoplasm? The CT?
|
The nuclei=blue
Cytoplasm=bright red CT=aqua blue/green |
|
|
In this technique, the tissue is impregnated with a reducible silver salt.
|
Silver Stain
|
|
|
Tissues or cells that stain with a silver salt are referred to as ?
|
argyrophilic
|
|
|
Nervous tissue and reticular fibers are often viewed with what kind of stain?
|
Silver stain
|
|
|
PAS=?
|
Periodic Acid Schiff
|
|
|
PAS stains what?
|
Stains glycogen and various carbohydrate containing molecules that contain an aldehyde group.
Appear dark magenta. |
|
|
The mucin produced by goblet cells as well as basement membranes stain magenta with what stain?
|
PAS
|
|
|
Why do basement membranes stain with PAS?
|
because of their proteoglycans
|
|
|
This technique is commonly used to look at the conditioni of the Bowman's capsule in the cortex of the kidney.
|
PAS
|
|
|
This stain is usually reserved for blood cells.
|
Wright's Stain
|
|
|
In this stain:
Nuclei are dark blue-purple Erythrocytes are pink-red Eosinophilic granules are bright red Basophilic granules stain a deep blue-purple |
Wright's Stain
|
|
|
In these stains, the tissue takes on a different color than the dye.
|
Metachromatic stains
|
|
|
Heparin granules in mast cells staining reddish purple using a toluidine blue stain is an example of what?
|
Metachromatic staining
|
|
|
What is the goal of immunocytochemistry?
|
The goal is to visualize some component (antigen) in a tissue section by means of an antibody.
|
|
|
Polyclonal Immunocytochemistry
|
Polyclonal antibodies are directed against several antigens.
|
|
|
Monoclonal Immunocytochemistry
|
antibodies are directed against one antigen.
|
