Histology - 1

Front 1

embedding?

Back 1

light microscopy technique, invovled making the specimen hard and stiff enough to cut/dehydrate

Front 2

basic dye? acidic dye?

Back 2

basic dye is hematoxylin, acidic dye is eosin

Front 3

what color will protein stain?

Back 3

prtoeins are weak bases. this means that it will attract the acidic dye, which is eosin.

pink

Front 4

what does the PAS stain do? what color?

Back 4

it stains carbohydrate components - PINK

Front 5

What does metal impregnation do?

Back 5

colloidal deposition of metal salts in tissue, it's black because it blocks light

Front 6

microfilaments

Back 6

thinnest in diameter, made of actin

Front 7

microtubules

Back 7

largest of the filaments, made of tubulin

Front 8

intermediate filaments

Back 8

in-between size, no specific protein

Front 9

four types of tissue?

Back 9

epithelium, connective, muscle, nervous

Front 10

what usually lines a conduit?

Back 10

simple squamous tissue

Front 11

where is simple squamous tissue found in the eye?

Back 11

corneal endothelium

Front 12

where is simple cuboidal epithelium found in the ey?

Back 12

anterior lens epithelium, anterior myoepithelium of iris,
pigmented epithelium of ciliary body

Front 13

simple columnar epithelium, what does nucleus look like? examples?

Back 13

elongated----posterior pigmented epithelium of iris, non-pigmented epithelium of ciliary body

Front 14

how many layers of cells in pesudostratified? barrier?

Back 14

one-, barrier is getting better....

Front 15

non-keratinized stratified squamous, always ___

still have ____ in the outer surface cells?

Back 15

wet, visible nuclei

Front 16

stratified cuboidal only comes in ___ layers. the nuclei _______. Barrier?

Back 16

very good barrier, does not let things pass. nuclei are in distinct rws. always 2 layers

Front 17

stratified columnar, always ___ rows. Nuclei are in ___.

Back 17

2, distinct rows

Front 18

the core of a microvilli

Back 18

actin microfilaments

Front 19

what do microvilli look like with a light microscope?
function?

Back 19

a continuous layer

function to inrease surface area for absorption

Front 20

what is a stereocilia?

Back 20

a long microvilli, usually occurs in tufts

Front 21

true cilia, synonym, structure, function?

Back 21

kinocilia, made of microtubules 9 doublets of microtubules and a single double in the center

Front 22

cilia also grow out of basal bodies which are related to ____.

made of microtubules but the arrangement is different, how?

Back 22

centrioles 9 triplets of microtubules and nothing in the middle

Front 23

junctional complex is a specific term for ___ junctions that occurr in a very specifc arrangement. First ___, second ___, third ____. Mostly seen in _____.

Back 23

zonula occludens, zonula adherens, and third is a desmosome. cuboidal/columnar cells

Front 24

a triplet of terminal junctions is known as a

Back 24

terminal bar

Front 25

tight junction, aka

Back 25

zonula occludens, network of ridges and grooves.

it is an effective barrier

a complete 'belt' is formed around the cell with its various neighbors

Front 26

adhering junctions (zonula adherens), ___ between cells

Back 26

clear

Front 27

junctional complex is usually near

3 layers are

Back 27

the laminal surface

zonula occludens, zonula adherens, desmosome

Front 28

in adhering junctions, cell membranes do ______

the fuzzy plaque -

Back 28

NOT fuse

represents interactions of proteins of adhering junction with intermediate filaments of cytoplasm

fuzzy plaque?

Front 29

macula adherens , synonym?
describe, looks like what on EM?

Back 29

desmosome, has the heftiest proeteins

looks dark

Front 30

gap junctions have a ___ channel, what size molecules can pass?

Back 30

true -- small to medium

Front 31

is the basement membrane organized?

Back 31

no, it's amorphous

Front 32

lamina lucida

Back 32

the top layer of a basement membrane, adjacent to and produced by epithelial cells

Front 33

lamina densa
contains ____

This is_________

Back 33

collagen type 4
the TRUE basement membrane layer, (aka basal lamina)

Front 34

What is the only place you can find type IV collagen?

Back 34

the basal lamina ( lamina densa)

Front 35

hemidesmosome

Back 35

very strong, hooks onto EC matrix

Front 36

descement's membrance is special how?

Back 36

it's in the corneal endothelium and is one of the thickets basement membrance int he body

Front 37

bowman's membrance is

Back 37

a non-cellular layer associated with the true basement membrane of hte corneal endothelium