Histo:amelogenesis D1 2008

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3 COMPONENTS OF DEVELOPING TOOTH GERM

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1. DENTAL ORGAN
2. DENTAL PAPILLA
3. DENTAL SAC

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DURING DEVELOPMENT ITS CELLS DIFFERENTIATE INTO AMELOBLAST WHICH FORM ENAMEL

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INNER DENTAL EPITHELIAL LAYER

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CELL(S) CAN CONTINUE TO GROW WITHOUT DIFFERENTIATION

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1. INNER DENTAL EPITHELIAL LAYER
2. DENTAL ORGAN

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EARLY STAGE OF ENAMEL FORMATION

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ENAMEL SECRETION

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MAIN COMPONENTS OF ENAMEL ORGANIC MATRIX THAT ARE SYNTHSIZED AND SECRETED BY AMELOBLAST

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1. AMELOGENIN
2. ENAMELIN

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REASON ENAMEL IS UNIQUE THAN PREDENTIN OR OSTEOID

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DOES NOT HAVE A SECRETED LAYER OF UNMINERALIZED MATRIX

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A SHORT PERIOD IN WHICH AMELOBLAST CHANGE FROM SECRETORY CELL TO PERFORMING A TRANSPORT FUNCTION IN LATE AMELOGENESIS

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TRANSITIONAL STAGE OF ENAMEL FORMATION

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IN TRANSITIONAL STAGE OF ENAMEL NUMBER OF AMELOBLAST ARE SIGNIFCANTLY REDUCE BY

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APOPTOSIS- GENETICALLY PROGRAMMED CELL DEATH

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IN TRANSITIONALL STAGE OF ENAMEL MOST THE PROTEIN SYNTHESIZING ORGANELLES ARE ELIMINATED BY

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LYSOSOMAL ACTION

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LATE STAGE OF ENAMEL FORMATION

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ENAMEL MATURATION

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ENAMEL STAGE WHERE SELECTIVE LOSS OF ENAMEL PROTEIN OCCUR AND MASSIVE INFLUX OF CALCIUM AND PHOSPHATE PROVIDE GROWTH FOR HYDROXYAPATITE CRYSTALS ( HIGH INORGANIC CONTENT)

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LATE STAGE- ENAMEL MATURATION

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CELL THAT DERIVED AMELOBLAST

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INNER DENTAL EPITHELIAL LAYER OF THE DENTAL ORGAN

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INNER DENTAL EPITHELIAL LAYER BEGIN TO DIFFERENTIATE AT THE GROWTH CENTER LOCATED IN WHICH PORTION (S) OF A DEVELOPING TEETH AND CONTINUE IN CERVICAL DIRECTION

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1. CUSP TIP
2. INCISAL EDGES

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A PRIMARY ENAMEL START OUT AS SIMPLE CUBOIDAL CELL WITH CENTRALLY LOCATED NUCLEI AND BECOME COLUMNAR CELL

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INNER DENTAL EPITHELIAL LAYER

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AN INNER DENTAL EPITHELIAL LAYER CELL (COLUMNAR) WHICH ACQUIRE MORE PROTEIN SYNTHESIZING ORGANELLES

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PRE-AMELOBLAST

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A CELL WHICH INDUCES PRE-AMELOBLAST TO COMPLETE THEIR DIFFERENTIATION TO SECRETORY AMELOBLAST

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FIRST LAYER OF DENTIN FORMED

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A TALL COLUMNAR CELL WITH CONE SHAPED TOMES PROCESSES AT THEIR DISTAL ENDS, INTERDIGITATE WITH THE SURFACE OF NEWLY SECRETED ENAMEL GIVING A PICKET FENCE APPEARANCE

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SECRETORY AMELOBLAST

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A STRUCTURE THAT DETERMINE THE ORIENTATION OF THE NEWLY NUCLEATED HYDROXYAPATITE CRYSTAL

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TOMES PROCESS

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ORGANELLES FOUND IN THE SECRETORY AMELOBLAST

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1. WELL DEVELOPED GOLGI COMPLEX
2. MANY GRANULAR ENDOPLASMIC RETICULUM CISTERNAE
3. NUMEROUS MITOCHONDRIA
4. SECRETORY GRANULES

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JUNCTIONAL COMPLEX PRESENT AT THE DISTAL AND PROXIMAL END OF SECRETORY AMELOBLAST

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1. TIGHT JUNCTION
2. INTERMEDIATE TYPE
3. DESMOSOMES

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JUNCTIONAL COMPLEX PRESENT ALONG THE LATERAL SURFACE OF SECRETORY AMELOBLAST

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1. GAP JUNCTION
2. DESMOSOMES

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ABSENT OF THIS STRUCTURE INDICATE ENAMEL SECRETION IS NEAR ITS END AND BECOMES PRISMLESS

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TOMES PROCESSES DISAPPEAR

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LOCATION OF JUNCTIONAL COMPLEX AND TERMINAL BAR

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1. DISTAL END
2. PROXIMAL END

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WHAT HAPPEN WHEN ENAMEL REACHES ITS FULL THICKNESS IN THE TRANSITIONAL STAGE

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1. AMELOBLAST BECOME SHORTER CELL
2. CONTAIN MANY LYSOSOME AND AUTOPHAGIC VACUOLES
3. PROTEIN SYNTHESIZING ORGANELLE ELIMINATED

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AT WHICH STAGE IS THE NUMBER OF AMELOBLAST IS REDUCED BY AS MUCH AS 50%

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TRANSITIONAL AMELOBLAST STAGE

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LONGEST STAGE OF AMELOGENESIS, WHEN AMELOBLAST MODULATE B/W TWO TYPES DEPENDING ON THE MORPHOLOGY OF THEIR DISTAL END

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ENAMEL MATURATION

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2 TYPES OF MATURATION AMELOBLAST

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1. RUFFLE-ENDED
2. SMOOTH- ENDED

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A MATURE AMELOBLAST WHICH POSSES NUMEROUS DISTAL MICROVILLE CONSTITUTE 80%

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RUFFED -ENDED AMELOBLAST

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A MATURE AMELOBLAST WHICH HAVE SMOOTH DISTAL ENDS AND ACCOUNT FOR 20%

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SMOOTH-ENDED

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A STAGE BEFROR TOOTH ERUPTION AMELOBLAST LOSE THEIR DIFFERENTIATION AND TOGEHTER W/ OTHER LAYERS OF DENTAL ORGAN REMAIN ON THE ENAMEL SURFACE AS REDUCED ENAMEL (DENTAL EPITHELIUM)

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END OF ENAMEL MATURATION

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DURING MATURATION A SPACE OCCUR AFTER THE LOSS OF ITS ORGANIC MATRIX SEEM ON GROUND SECTION

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ENAMEL SPACE

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TRUE OR FALSE; DURING ENAMEL MATURATION MOST OF THE ENAMEL PROTEIN ARE LOST

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TRUE

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PROCESS WHEN HIGHLY MINERALIZED ENAMEL ARE LOST

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HISTOLOGICAL PROCESSING ( (DEMINERALIZATION)

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A STAGE ENAMEL PROTEIN ARE SECRETED AND PARTIALLY MINERALIZED

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EARLY ENAMEL STAGE

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CHARATERISTICS OF LATE MATURATION STAGE

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1. MASSIVE AMOUNT OF CALCIUM AND PHOSPHATE DEPOSITED
2. SELECTIVE LOSS OF ENAMEL PROTEINS AND WATER OCCUR

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AN ENAMEL THAT REMAIN AFTER A ENAMEL IS FULLY FORMED, THE AMELOBLAST LOSE THEIR DIFFERENTIATION

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REDUCED ENAMAL EPITHELIUM