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41 Cards in this Set
- Front
- Back
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Gram stain of E. coli
on mac? on ba? |
Gram neg rods
Lactose fermentor Variably hemolytic |
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Acinetobacter
-gram stain -on MAC? |
-gram neg rod
-nonfermentor |
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P. aeruginosa
-gram stain -on mac |
Gram neg rod
-nonfermentor, turns green and smells like grape |
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How to differentiate P. aeruginosa from E. coli
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Oxidase pos
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How does P. aeruginosa make viscous sputum?
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Alginate capsule
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B. subtilis
-gram stain |
Gram pos rod
-endospores |
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Does B. subtilis grow on MS?
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Yes; but not a mannitol fermentor.
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Corynebacteria
-gram stain |
PLEOMORPHIC gram pos rods
-palisades -clubs -angular |
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What species makes the teeniest colonies on BA?
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Coryne xerosis
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Why do Corynebacterium need BA?
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they're fastidious
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How is C. diphtheria different from the other species?
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Beta hemolytic
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What are the 3 corynebacteria that we did stuff with in lab?
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C. diphtheria
C. xerosis C. pseudodiphtheriticum |
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What did we compare the Corynes to?
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Listeria monocytogenes
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What do C. xerosis and C. pseudodiphtheriticum have in common? (4 tests)
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-Reduce nitrate
-Catalase pos -Nonhemolytic -Bile esculin neg |
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What tests differentiate C. xerosis and C. pseudodiph?
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-C. xerosis Ferments Glucose in CTA (yellow; Urease NEG
-C. pseudodiph does not ferment glucose (red); Urease POS |
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Listeria monocytogenes:
-catalase -hemolysis -BE -Urea -Nitrate -Fermentation in Glucose CTA? |
-Weakly cat +
-Hemolytic (like C. diph) -BE POS -Urease neg -Nitrate neg -Fermentor (yellow) |
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Haemophilus species
-gram stain |
Gram neg coccobacilli
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Nutrient requirements of haemophilus:
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-X factor (hemin)
-V factor (NAD) |
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Nutrient factor for H. influenzae; hemolysis?
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X and V
nonhemolytic |
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Nutrient factor for H. parainfluenzae; hemolysis?
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V only
nonhemolytic |
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Nutrient factor H. haemolyticus;
hemolysis? |
X and V
hemolytic |
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Nutrient factor for H. parahaemolyticus; hemolysis?
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V only
hemolytic |
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What tests differentiate Strep A from Strep B? Results?
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Bacitracin
Strep A = sensitive Strep B = resistant Agglutination = Strep B only |
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Test for Enterococci:
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-Grows in 6.5% NaCl
-BE positive |
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What tests differentiate Strep pneumo from Strep viridans?
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-Optichin
Strep pneumo = sensitive Strep viridans = resistant Agglutination: Strep pneumo |
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Which 2 streps are positive for agglutination?
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-Strep group B
-Strep pneumo |
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Pigmentation of Staph aureus on agar:
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Golden to cream
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Pigmentation of Staph epi on agar:
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White to gray
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Neisseria gram stain:
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Kidney bean shaped gram neg cocci in pairs (think of n. gonorrhoea on direct smear)
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Distinctive characteristic of Neisseria:
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oxidase positive
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Which test is N. gonorrhoea positive for?
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PEP pos
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What is the PEP test?
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Proline hydrolysis - it releases free naphthylamide
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What color is naphthylamide?
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red
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What enzyme does N. meningitidis produce?
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y-glutamylaminopeptidase
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What results from N. meningitidis's enzyme?
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Release of nitroanilide base
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What color is the nitroanilide base?
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Yellow
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How do you confirm N. meningitidis?
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Add PEP - turns blue or purple
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What enzyme does N. lactamica produce?
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Beta galactosidase
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What color does N. lactamica turn the Kwik test?
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Blue
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What enzyme does Moraxella catarrhalis produce?
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Butyrate esterase
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What test detects M. catarrhalis? What is the result?
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CATscreen; light blue (pos)
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