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62 Cards in this Set
- Front
- Back
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CLL/SLL:
CD10 Bcl6 Bcl2 CD5 CD23 CD43 |
CLL/SLL:
CD10: - Bcl6: - Bcl2: + CD5: + CD23: + CD43: +/- |
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Mantle Cell Lymphoma:
CD10 Bcl6 Bcl2 CD5 CD23 CD43 Cyclin D1 |
Mantle Cell Lymphoma:
CD10: - Bcl6: - Bcl2: + CD5: + CD23: - CD43: +/- Cyclin D1: + |
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Follicular Lymphoma
CD10 Bcl6 Bcl2 CD5 CD23 CD43 |
Follicular Lymphoma:
CD10: + Bcl6: + Bcl2: + CD5: - CD23: - CD43: - |
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Lymphoplasmacytic Lymphoma
CD10 Bcl6 Bcl2 CD5 CD23 CD43 CD138 |
Lymphoplasmacytic Lymphoma
CD10: - Bcl6: - Bcl2: +/- CD5: - CD23: - CD43: +/- CD138: + |
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Hairy Cell Leukemia
CD10 Bcl6 CD5 CD23 CD25 TRAP CD103 (flow) CD11c (flow) |
Hairy Cell Leukemia
CD10: - Bcl6: - CD5: - CD23: - CD25: + TRAP: + CD103 (flow): + CD11c (flow): + |
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For Hairy Cell Leukemia, what stains would you order and what would be the expected results? Flow pattern?
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Stains: CD10-, Bcl6-, CD5-, CD23-, CD25+, TRAP+, CD20/22+
Flow: CD20 bright, CD22 bright, CD103+, CD11c+, CD25+ |
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Translocation in Follicular Lymphoma?
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Follicular Lymphoma:
t(14;18) 14 = IGH 18: BCL2 |
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Translocations in Burkitt lymphoma?
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Burkitt Lymphoma:
t(8;14) t(8;22) t(2;8) 8 = c-MYC 14 = IGH 2 = kappa 22 = lambda |
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Burkitt lymphoma IHC?
CD3 CD20 CD10 Bcl6 Bcl2 Ki67 |
Burkitt Lymphoma:
CD3: - CD20: + CD10: + Bcl6: + Bcl2: - Ki67: 100% |
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How is germinal center (GC) diffuse large B cell lymphoma (DLBCL) differentiated from non-GC DLBCL by CD10, Bcl6, and MUM1?
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GC:
CD10+ CD10-, BCL6+, MUM1- Non-GC: CD10-, Bcl6+, MUM1+ CD10-, Bcl6- |
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Translocation in Mantle Cell Lymphoma?
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Mantle Cell Lymphoma:
t(11;14) 11 = CCND1 (cyclin D1 = Bcl-1) 14 = IGH |
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IHC for thymic large B-cell lymphoma?
CD45 CD30 MUM1 CD23 |
Thymic Large B-cell Lymphoma:
CD45+ CD30+ MUM1+ CD23+ |
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Translocation in Anaplastic Large Cell Lymphoma (ALCL)?
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Anaplastic Large Cell Lymphoma:
t(2;5) 2 = ALK 5 = NPM (nucleophosmin) |
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Which type of low-grade B-cell lymphoma infiltrates the bone marrow in a paratrabecular pattern? (2)
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Follicular lymphoma
Mantle cell lymphoma can also do this. |
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Which type of low-grade B-cell lymphoma infiltrates the bone marrow in a diffuse pattern with "fried egg" cytology?
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Hairy cell leukemia
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Which type of low-grade B-cell lymphoma infiltrates the bone marrow with random nodules?
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Marginal zone lymphoma
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What substance makes up basophilic stippling?
What substance makes up Howell-Jolly bodies? What substance makes up Heinz bodies? What substance makes up Pappenheimer bodies? |
Basophilic stippling: ribosomal RNA
Howell-Jolly bodies: DNA Heinz bodies: denatured hemoglobin Pappenheimer bodies: iron |
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What lymphoma?
CD20+, CD3-, CD10-, CD5-, CD23-, CD22+, CD25+, Ki-67 not high |
Hairy cell leukemia
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Clinical manifestation of hairy cell leukemia? (2 things)
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splenomegaly, neutropenia
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Which lymphoma?
CD20+, CD3-, CD5+, CD23+, cyclin D1-, Ki67 not high |
CLL/SLL (chronic lymphocytic leukemia/small lymphocytic lymphoma)
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In CLL/SLL, the following characteristics infer good or bad prognosis:
- diffuse marrow involvement - increased proportion of prolymphocytes (over paraimmunoblastic) - trisomy 12, thus, predominantly unmutated immunoglobulin variable gene regions - 13q14 abnormalities, thus, mutated immunoglobulin variable gene regions - CD38+, ZAP70+ - CD38-, ZAP70- |
- diffuse marrow involvement: poor
- increased proportion of prolymphocytes (over paraimmunoblastic): poor - trisomy 12, thus, predominantly unmutated immunoglobulin variable gene regions: poor - 13q14 abnormalities, thus, mutated immunoglobulin variable gene regions: good - CD38+, ZAP70+: poor (naive B cell type) - CD38-, ZAP70-: good (post germinal center) |
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The lesional cells are medium-sized lymphoid cells with round to oval nuclei and condensed chromatin pattern with deep blue vacuolated cytoplasm.
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Burkitt lymphoma
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Which leukemia?
CD11c+, CD64+, CD33+, CD13+ |
Monoblastic leukemia (M5)
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Which leukemia?
CD19+, CD10+, CD34+, IgM+ |
Pre B-ALL
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Which leukemia?
CD19+, CD20+, CD11c+, CD103+ |
Hairy cell leukemia
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Which leukemia?
CD34-, HLA-DR-, CD33+, MPO+ |
Acute promyelocytic leukemia (M3)
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Which lymphoma?
CD19+, CD20+, CD10+, BCL2+ |
Follicular lymphoma
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What's the association between progressive transformation of germinal centers (PTGC) and Hodgkin lymphoma?
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A small fraction of PTGC is associated with NLP-Hodgkin but most cases of PTGC have no lymphoma association. PTGC is not associated with classic HL.
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H. pylori is to gastric MALT lymphoma
as _______ is to cutaneous extranodal marginal zone lymphoma |
Borrelia burgdorferi
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Translocation in ALCL?
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Anaplastic Large Cell Lymphoma:
t(2;5) 2 = ALK 5 = NPM (nucleophosmin) |
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Which type of cells best fits with this flow pattern?
CD4+/CD8-, CD4-/CD8+, CD4+/CD8- |
Thymic T cells
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What is the CD4/CD8 expression of NK cells and cytotoxic T cells?
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CD4-/CD8+
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What chromosome is MLL on?
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11q23
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Translocation in APL (M3)?
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Acute Promyelocytic Leukemia:
t(15;17) 15 = PML 17 = RARA |
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Translocation in AML with abnormal eosinophils (M4Eo)?
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M4Eo
inv(16) t(16;16)(p13.1;q22) CBFB-MYH11 rearrangement |
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Translocation in AML with granulocytic maturation (M2)?
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AML with Granulocytic Maturation (M2)
t(8;21) 8 = RUNX1T1 = ETO 21 = RUNX1 = AML1 |
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Ring granulomata in bone marrow is characteristic of what infection caused by what organism?
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Q fever, Coxiella burnetii (Gram negative bacterium)
The story: you get Q fever by breathing in spore-like cell variant from contact w/ animals (e.g. cat, dog, goat, cattle, sheep) and results in flu-like Sx or atypical PNA 2 weeks after exposure. |
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Translocation in acute monoblastic leukemia (M5a) or monocytic leukemia (M5b)?
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Acute Monoblastic Leukemia (M5a) or Monocytic Leukemia (M5b):
t(9;11) 9 = MLLT3 11 = MLL |
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Do the following have good or bad prognosis in B lymphoblastic leukemia (pre-B-ALL):
t(9;22)(q34;q11.2) t(v;11q23) t(12;21)(p13;q22) hyperdiploidy hypodiploidy t(5;14)(q31;q32) t(1;19)(q23;p13.3) |
Pre-B ALL:
t(9;22)(q34;q11.2): bad 9 = ABL 22 = BCR t(v;11q23): bad 11 = MLL t(12;21)(p13;q22): good 12 = ETV6 = TEL 21 = RUNX1 = AML1 hyperdiploidy = over 50 chromosomes: good hypodiploidy: bad t(5;14)(q31;q32): uncertain (too few cases) 5 = IL3 14 = IgH t(1;19)(q23;p13.3): bad 1 = PBX1 19 = E2A = TCF3 |
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In pre-B-ALL what translocations are associated with:
Infant Child Adult |
Infant: t(4;11)
4 = AF4 11 = MLL Child: t(12;21) 12 = ETV6 = TEL 21 = RUNX1 = AML1 Adult: t(9;22) 9 = ABL 22 = BCR |
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What type of lymphoma is described below?
Extranodal and systemic neoplasm derived from cytotoxic T-cells usually with gamma-delta receptor rearrangement. These neoplasms are almost always associated with isochromosome 7q. The neoplastic cells are usually medium-sized demonstrating marked sinusoidal infiltration of spleen, liver, and bone marrow. Patients may be immunosuppressed presenting iwth hepatosplenomegaly, systemic symptoms, and pancytopenia. |
hepatosplenic T-cell lymphoma
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Which lymphoma is being described?
Cytotoxic T-cell lymphoma, which preferentially infiltrates subcutaneous tissue. It is composed of atypical lymphoid cells of varying size, typically with karyorrhexis of tumor cells and associated fat necrosis. This neoplasm may be associated with hemophagocytic syndrome. |
Subcutaneous panniculitis-like T-cell lymphoma
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Which type of lymphoma is being described?
A peripheral T-cell lymphoma most often composed of highly pleomorphic lymphoid cells. The disease is usually widely disseminated and is caused by the human retroviurs, human T-cell lymphoma virus type 1 (HTLV1). The phenotype is CD3+, CD5+, CD4+, CD8-. The most common acute variant is associated with hypercalcemia with or without lytic bone lesions. |
Adult T-cell leukemia/lymphoma
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Which type of lymphoma is being described?
This neoplasm is epidermotropic, characterized by infiltrates of small to medium-sized T cells with cerebriform nuclei. This is the most common subtype of T-cell lymphoma that arises primarily in the skin. The phenotype is CD3+, CD5+, CD4+, CD8-. |
mycosis fungoides
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What syndrome is being described?
Defined by the triad of erythroderma, generalized LAD, and clonal T cells with cerebriform morphology found in skin, lymph nodes, and peripheral blood. Also, one or more of the following is required: 1. absolute neoplastic cell count of 1000 cells per mm3 2. CD4/CD8 ratio >10 3. loss of one or more T-cell antigens |
Sezary syndrome
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Extraosseous plasmacytoma most commonly involves what area?
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Upper respiratory tract (80%)
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Most cases of multiple lymphomatous polyposis (multiple, variably sized polyps showing lymphoma) represent what?
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Mantle cell lymphoma
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Which lymphoma is associated with proliferation of centrocyte-like cells?
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Mantle cell lymphoma
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Which lymphoma is characterized by starry sky appearance?
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Burkitt lymphoma
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A proliferation of a mixture of centorcytic and centroblastic cells defines what entity?
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Follicular lymphoma
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Proliferation of paraimmunoblastic cells is is seen in what entity?
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SLL/CLL
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Intrasinusoidal pattern of infiltration is typical of what entity?
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ALCL
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What disease is being described?
Leukocytosis, organomegaly (due to tissue infiltration by leukemic cells), thrombocytosis, varying degrees of myelofibrosis. |
CML
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Extranodal marginal zone lymphoma is most commonly found where?
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stomach
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Gaucher's disease is due to a mutation in the gene for what enzyme?
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glucocerebrosidase
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What is the most common lysosomal storage disease?
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Guacher's disease
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The gene for glucocerebrosidase is mutated in what disease?
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Gaucher's disease
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What product is accumulated in Gaucher's disease?
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glucosylceramide
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Glucosylceramide is accumulated in what disease?
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Gaucher's disease
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In Gaucher's disease, glucosylceramide accumulates in what cells of the body?
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Phagocytic cells.
Note: in some forms glucosylceramide accumulates in the CNS. |
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In what organs do Gaucher cells accumulate? (6)
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liver, spleen, lymph nodes, thymus, tonsils, and bone marrow
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What are the fibrillary structures which give Gaucher cells the "tissue paper" quality?
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By EM, they are elongated, distended lysosomes rich in the stored sphingolipid, glucosylceramide.
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