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20 Cards in this Set

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What is the reference range for RBC for males and females

4.7 - 6.1 x 10^6/ul (females)




4.2 - 5.4 x 10^6/ul (males)

What is the reference range for WBC for adults

4.8 - 10^3/ul

What is the reference for PLTs for adults

150 - 400 x 10^9/L

What is the primary choice of collection

EDTA (Purple Top)

a tripotassium salt




inhibits coagulation by chelating the binding of Ca ions




preserves cellular morphology and prevents platelet aggregation




increases cell shrinkage

EDTA

What is the second choice of collection

NaCit (Blue top tube)

PLATELET ONLY




binds Ca into a soluble complex




DO NOT report any other parameters




Multiply by 1.1

NaCit

What is the third choice of collection

ACD (yellow top)

Platelet only




usually used to obtain accurate platelet count when clumps seen with EDTA




DO NOT report any other peramiters




Mulitply results by 1.17

ACD

10 - 16 um




2-5 lobes (usually 3)




lobes connected by at least one filament




Nucleus: clumped-coarse, puplish red




Cytoplasm: stain pale pink to neutral, 2nd granuales



Segmented Neutrophils

1st line of defense against infection

Segmented Neutrophils

7-15 um




Nucleaus: dark purple, condensed chromatin, tends to clump on outside of cell




Cytoplasm: Blue, may contain purplished-red

Lymphocytes

Types of Lymphs

T-clls, B-cells, Natural killer cellls

12-18 UM




Nucleus: folded, indented, convoluted, lacy chromatin with linear clumping




Cytoplasm: dull gray-blue, fine small




N:C= 1:1 or 2:1




digestive vacuoles

Monocyte

Circulates in the blood for 8h to 3d




transforms into macrophages when in the tissues




Chemotaxis, pagocytosis, Ag presentation, release of cytokines

Monocytes

10-16 um




Nucleus: 2-3 lobes, condensed chromatin




Cytoplasm: orange/reddish-orange granules




High in nights, low in mornings

Eosinophil

10-16 um




Cytoplam is pale blue to colorless




2-3 lobes




chromatin is loose but clumps in bailed pattern




large blue-black granules fill the cytoplasm

Basophil

7-8 um




15-2.5 um




Biconcave disc




Pinkish hue




No nucleus




Transports O2 and excrete CO2

Erythrocyte

what is the thinnest part of the RBC

Central pallar

1-4 um




Reddish-purple granules in a small amount of bluish cytoplasm




No nucleus




Norm Av 7-15 PLT/OIF




Function: Maintain Hemostasis; Adhere

PLT (Thrombocyte)