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18 Cards in this Set
- Front
- Back
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often preferred to the powdered form because it mixes more easily with blood |
liquid tripotassium EDTA |
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occurs when platelet agglutinates are similar in size to WBCs and automated analyzers cannot distinguish the two |
pseudoleukocytosis |
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most convenient and commonly used method for making peripheral blood films |
manual wedge technique |
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angle used in spreading |
30- to 45- degree angle |
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blowing breath on a slide is counterproductive because the moisture in breath causes RBCs to become |
echinocytic |
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used for staining peripheral blood films and bone marrow smears |
wright-giemsa stain |
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fixes the cells to the slide |
methanol |
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it is basic and stains acidic |
free methylene blue |
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is tis acidic and stains basic |
free eosin |
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the buffer that is added to the stain should be |
0.5 M sodium phosphate (pH 6.4) |
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appearance that should appear on the slide if mixing is correct |
metallic sheen (or green "scum") |
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refers to the presence of more than four times the number of cells per field at the edges indicates that the film is unacceptable |
snowplow effect |
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preferred pattern used in reading the film |
back and forth serpentine battlement track pattern |
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may be reported as a separate percentage of the100 cells, as a percentage of the total number of lymphocytes |
reactive lymphocytes |
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formula for platelet estimate |
average # of platelets per field x total RBC count/ 200 RBCs per field |
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the numbers and descriptions generated by the testing are summarized using appropriate terminology |
Phase 1 |
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recognize a pattern of results consistent with various diseases and to be able to narrow the diagnosis for the given patient or perhaps even to pinpoint it |
phase 2 |
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peripheral blood films and bone marrow should be evaluated in a |
systematic manner |
