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32 Cards in this Set
- Front
- Back
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-deficiency of all three cellular components of the blood (red, white cells and platelets) -may lead to BM failure |
pancytopenia |
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-general term for BM failure -most commonly acquired due to toxic substance (benzene, chloramphenicol, lead) |
aplastic/ hypoplastic BM |
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due to congenital disorders or chromosomal breaks |
congenital aplastic anemia or constitutional aplastic anemia |
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common SPECIAL stain to detect presence of iron (blue) in biopsy specimens |
perls' stain/ prussian blue reaction |
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Bm produces ringed sideroblasts rather than healthy RBC |
sideroblast anemia |
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-hereditary disorder in which iron salts are deposited in the tissues leading to liver damage, Dm and bronze discoloration of skin -excessive iron overload in the body |
hemochromatosis |
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subgroup of lipid storage disorders called sphingolipidoses in which harmful quantities of fatty substances or lipids accumulate in the spleen, liver, lungs, BM and brain |
Niemann-pick disease |
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rare genetic disorder characterized by the deposition of glucocerebroside in cells of the macrophage-monocyte system |
gaucher disease |
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deficient enzyme in gaucher's disease |
glucocerebrosidase |
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vitamin k deficiency -clotting factors |
2 7 9 10 |
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how to treat vitamin k deficiency |
green leafy vegetables lactobaccillus e. coli |
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ideal for adults -both aspiration and core biopsy |
pelvic bone |
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-adults and children -not prone to injury -yields aspirate and core biopsy |
posterior superior iliac crest |
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for patients who cant lie sideways -same with posterior superior iliac crest |
anterior superior iliac crest |
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-ideal site for children (less than 2 yrs old) -anterior medial surface of the tibia -USED ONLY for aspiration |
tibia |
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-prone to heart injury -provides ample material for aspiration but is only 1 cm thick and CANT be used for core biopsy |
sternum |
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rarely used unless they are the site of a suspicious lesion discovered on a radiograph |
spinous process of the vertebrae, ribs or other red marrow containing bones |
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process wherein a bone is subjectred to perforation/ puncture using a trephine needle, jamshidi needle or weterman-jensen needle |
trephination |
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bone itself is removed |
core biopsy |
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how much bone marrow fluid is req for BM aspirate |
1 to 1.5 mL |
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-demonstrates bone marrow architecture -for estimation of BM cellularity -MUST be collected first |
core biopsy |
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-5 to 10 slides -imprint preparations may imitate aspirate morphology |
imprints/ touch preparation |
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fixative after transfer |
methanol - 80 to 100% |
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to preserve the cells/ tissue and prevent autolysis |
fixation |
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fixatives which may be used |
- 5 to 10% buffered neutral formalin -zenker's solution -B5 - fixative of choice |
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routine stain used for histo technique lab |
hematoxylin and eosin (H&E) |
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routine for bone marrow film |
Pearl's prussian blue (ferric ferricyanide) iron stain |
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aka modified romanofsky stain |
wright or wright-giemsa stains |
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-used to evaluate cellularity and hematopoietic cell distribution -locate abnormal cell clusters |
hematoxylin and eosin |
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-used to evaluate iron stores for deficiency or excess iron |
prussian blue (ferric ferricyanide) iron stain |
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preferred for iron store estimation
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aspirate smear or EDTA acid chelation |
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used to observe hematopoietic cell structure *cell identification is less accurate in a biopsy specimen than in an aspirate smear |
wright or wright-giemsa dyes |
