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19 Cards in this Set
- Front
- Back
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What is the cofactor of all kinases used in glycolysis?
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Mg2+
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What is the 1st step in Glycolysis?
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Glucose + (Hexokinase/Mg2+) --ATP--> Glucose-6 phosphate +ADP
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What is Step 2 of Glycolysis?
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Glucose-6 phosphate + Phosphoglucose Isomerase ---> Fructose-6 phosphate
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What is step 3 of Glycolysis?
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Fructose-6 phosphate + Phosphofructokinase/Mg2+ --ATP--> Fructose-1,6 bisphosphate + ADP
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What is step 4 of Glycolysis?
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b-D-Fructose-1,6 bisphosphate + Fructose Bisphosphate Aldolase ----> dihydroxyacetone phosphate and glyceraldehyde 3-phosphate.
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Why put a phosphate on a glucose?
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1. It "locks" the glucose in the cell, since there are no phosphoglucose channels.
2. It keeps the concentration of free glucose in the cell low, so that more keeps flowing in. |
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At what point does glycolysis effectively become "irreversible"?
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Step 3, yielding Fructose 1,6-bisphosphate.
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Why is Step 3 considered irreversible?
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The energy put into it destabilizes the molecule, forcing the rxn forward.
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What is step 6 of Glycolysis?
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glyceraldehyde 3-phosphate + glyceraldehyde phosphate dehydrogenase ---Pi--NAD+--> 1,3-bisphosphoglycerate + NADH
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What is Step 5 of glycolysis?
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Dihydroxyacetone phosphate + triosephosphate isomerase -----> D-glyceraldehyde 3-phosphate
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What is the 7th step of glycolysis?
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1,3-Bisphosphoglycerate + Phospholycerate Kinase/Mg2+ ---ADP---> 3-Phosphoglycerate + ATP
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What is the 8th step of glycolysis?
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3-Phosphoglycerate + Phosphoglycerate Mutase ----> 2-Phosphoglycerate
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What is he 9th step in glycolysis?
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2-Phosphoglycerate + Enolase/Mg2+ -----> Phosphoenolpyruvate
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What's the final step in Glycolysis?
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Phosphoenolpyruvate + Pyruvate Kinase/Mg2+ --ADP--> Pyruvate + ATP
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Describe regulation of glycolysis via hexokinase/ glucokinase.
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When [glucose-6-phosphate] are high, hexokinase's activity is allosterically inhibited; hexokinase works best at low [glucose]. When [glucose] is high, glucokinase is activated and its glucose-6-phosphate product is stored as glycogen.
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Which steps yield ATP?
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Steps 7 & 10
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Which step yields reduced NADH?
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Step 6
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What is the net yield from 1 glucose in glycolysis?
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2 ATP, 2 NADH, 2 pyruvte
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What are the possible fates of pyruvate?
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Fermentation or enters the TCA cycle.
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