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10 Cards in this Set

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Give the Results for Salmonella:

-MaConkey

-XLD Agar

-Hektoen

-TSL
**Salmonella**
No lactose fermentation, Alkaline slant,black deep, **H2S PRODUCER**

-MaConkey-
CLEAR--> no lactose fermentation

-XLD Agar-
BLACK --> H2S --> FeS

-Hektoen-
BLACK --> H2S, no carbon fermentation

-TSL-
RED over BLACK
(Red=glucose fermenter, Black=H2S)
Give the results for Shigella:

-MaConkey

-XLD Agar

-Hektoen

-TSL
***Shigella***
Non-lactose fermentation is classic. Alkaline slant, acid deep with No H2S

-MaConkey-
CLEAR --> NO LACTOSE FERMENTATION

-XLD Agar-
CLEAR-->no xylose, sucrose or lactose fermentation

-Hektoen-
CLEAR--> no sucrose, lactose or salicin fermentation

-TSL-
RED over YELLOW
(Red=only uses glucose, YELLOW=no H2S)
Give the results for 0157 E.coli:

-MaConkey Sorbitol

-MaConkey

-XLD Agar

-Hektoen

-TSL
***0157 E.Coli***
SORBITOL NEGATIVE, xylose and LACTOSE FERMENTING. Acid slant, acid deep, H2S negative

-MaConkey Sorbitol-
CLEAR--> SORBITOL (-)

-MaConkey-
PINK-->LACTOSE FERMENTER

-XLD Agar-
YELLOW-->xylose, sucrose or lactose fermentation

-Hektoen-
YELLOW-->sucrose, lactose or salicin fermentation

-TSL-
YELLOW over YELLOW
(ferments all sugars, no H2S)
This organism often contaminates beef. Most cases involve undercooked hamburgers
0157 E.coli

Patient will be nauseated but NOT VOMITING. Watery diarrhea turns to bloody diarrhea
Why are antibiotics contraindicated in 0157 E.coli?
ABX are thought to lyse the organism and release shiga toxin into pt.

Supportive treatment is recommended
Pathogenesis:
Invasion of M cells by means of ACTIN

Stool will have mucous and blood streaks

Most often spread fecal-orally
Shigella
-Can be picked up by WBCs and transferred to lymph nodes where it can enter blood and eventually seed in gall bladder

-Often contaminates chicken or other food products
Salmonella
What is a Kirby Bauer Susceptibility study?
Uses diameter of zone of inhibition to determine if drug is sensitive of resistant
What is Minimal Inhibitory Concentration (MIC)?
Measures the lowest concentration of antibiotic that inhibits the growth of test organisms
What is the relationship b/t Kirby Bauer Susceptibility and Minimal Inhibitory Concentration studies?
INVERSE RELATIONSHIP between Size of Kirby Bauer inhibition zone and MIC

(Larger zone = smaller MIC)