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25 Cards in this Set
- Front
- Back
- 3rd side (hint)
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their proposed structure
for DNA |
In 1953, James Watson
and Francis Crick unveiled |
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Mendel’s “heritable factors”
Morgan’s genes on chromosomes |
The DNA structure that
had been the major player in both: |
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James Watson and Francis Crick
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They were awarded the
Nobel prize in 1962 |
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the
structure of DNA |
There were several important
contributions made to allow Watson and Crick to come to their conclusion on |
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Hershey and Chase
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nucleotides contain
“programming” information |
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Chargaff
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nucleotide composition
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Rosalind Franklin
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x-ray crystalography
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Alfred Hershey and Martha Chase
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who found that DNA is the molecule transferred from virus to bacteria to infect (reprogram) the cell
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Erwin Chargaff
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analyzed
the nucleotide base composition of a variety of organisms |
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(#1) ratios
(#2) the concentrations |
He found that (blank#1) were
diverse among organisms He found a relationship between (blank#2) |
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Rosalind Franklin
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is credited with providing the
evidence for the symmetry of DNA, and was essential to Watson and Crick’s conclusions |
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Rosalind Franklin
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She died in 1958 from
cancer, and thereby was not included as a Nobel laureate |
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Watson and Crick
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took all these pieces of
evidence and finally made the jump to how the base paring and structure worked in DNA |
2 people
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the replication
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After the structure was deduced, the next step
was |
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semiconservative
no evidence to support the idea |
Watson and Crick suggested that the replication was
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Matthew Meselson and
Franklin Stahl |
The evidence was provided in 1958 by
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E. coli
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Matthew Meselson and
Franklin Stahl radioactively labeled DNA and followed it in |
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All DNA is
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Complementary
A only with T G only with C Anti-parallel Runs from 5’ to 3’ Read and synthesized only in this direction |
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nucleotide pairs (base pairs)
In one chromosome |
E. coli has about 46 million
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46 chromosomes
~ 6 billion base pairs Typed like a textbook would fill 1200 books |
Humans have (blank) chromosomes
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hours
Only one error per 10 billion bases |
Your DNA is copied in a few
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10,000
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One gene (for one protein) is about (how many) base pairs
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origins of
replication (ori) 1 in bacteria 100-1000 in eukaryotes |
DNA replication starts
at the |
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Limits of DNA
polymerase: |
Can only add
nucleotides to an existing strand Can’t initiate strand Can only add nucleotides in one direction 5’ to 3’ (new strand) |
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A leading strand and a lagging strand
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Must be synthesized in small chucks, and then connected
Chucks called Okazaki fragments |
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