Genetics 2

Front 1

What are the 4 major characteristics of genetic material?

Back 1

1. Replication
2. Information Storage
3. Expression of Information
4. Variation by Mutation

Front 2

What does the initial event, Transcription, result in the synthesis of?

Back 2

1. mRNA
2. tRNA
3. rRNA

Front 3

____ are transferred into proteins.

Back 3

mRNA's

Front 4

Fill in the following:
___-->(TRANSCRIPTION)-->___,___,___ ==>ribosome--->(TRANSLATION-->___.

Back 4

DNA
mRNA, tRNA, rRNA
Protein

Front 5

Who?
"The transforming substance is DNA."

Back 5

Avery, MacLeod, McCarty

Front 6

Who?
"Some substance from dead cells (of a virulent strain) is able to transform avirulent cells into virulent cells."

(Transformation in Streptococcus pneumoniae)

Back 6

Fred Griffith

Front 7

Who?
"DNA, and not protein, is responsible for directing the reproduction of phage T2 during infection of E.Coli."

Back 7

Hershey and Chase

Front 8

A.)The amount of DNA in a cell is correlated to the ____ level of a cell.

B.)No such correlation exists fo ____.

Back 8

A.)Ploidy
B.)Proteins

Front 9

_______ demonstrated that DNA replicates in a semiconservative manner.

Back 9

Meselson and Stahl

Front 10

In reasonably short time, many new phages are constructed and the bacterial cell is lysed, releasing the progeny viruses.

Back 10

Lytic Cycle

Front 11

T2 Phage consist of approx. ___% Protein and ___%DNA.

Back 11

50% Protein
50% DNA

Front 12

Infection is initiated by adsorption of the phage by its ____ to the bacterial cell.

Back 12

tail fibers

Front 13

DNA is the substance responsible for ____________.

Back 13

Bacterial Transformation

Front 14

The production of new viruses occurs within the ______ cell.

Back 14

Bacterial

Front 15

Genetic material in phage T2 is _____, not protein.

Back 15

DNA

Front 16

Consists of long, parallel fibers wrapped around each othe in a helical fashion.

Back 16

DNA

Front 17

The process of infection by only the viral nucleic acid.

Back 17

Transfection

Front 18

UV light that is most mutagenic is absorbed maximally by ___, but not by ___.

Back 18

DNA
Protein

Front 19

T/F
In DNA, A=T and G=C but A+T does NOT equal G+C. (But A+G=T+C)

Back 19

TRUE!

Front 20

If A=T and G=C, then A+_=T+_

Back 20

A+G=T+C

Front 21

During DNA denaturation, ____ bonds are broken.

Back 21

Hydrogen

Front 22

DNA is found only where primary ____ function occurs.

Back 22

Genetic

Front 23

Protein is found ____ on the cell.

Back 23

Everywhere

Front 24

DNA and RNA absorb UV light most strongly at ____nm.

Protein absorbs most strongly at ___nm.

Back 24

260nm
280nm

Front 25

DNA is a nucleic acid and _______ are the building blocks of all nucleic acid molecules.

Back 25

Nucleotides

Front 26

What 3 essential compounds do nucleotides contain?

Back 26

1.Nitrogenous base (2 types)
2. Pentose sugar (5 carbon sugar)
3. Phosphate group

Front 27

Name the 2 types of Nitrogenous bases.

Back 27

1.Purines (nine-member double ring--2 types
2.Pyrimidine (six-member single ring)--3types

Front 28

A&G are _____
C, T and are _____.

Back 28

A&G=PURINE
CT&U=Pyrimidine

Front 29

If a molecule is composed of a purine or pyrimidine base and a ribose or deoxyribose sugar, the chemical unit is called a ______.

Back 29

Nucleoside

Front 30

If a phosphate group is added to a nucleoside, the molecule is called a _________.

Back 30

Nucleotide

Front 31

What is the difference between a nucleoside and a nucleotide?

Back 31

Nucleosides don't have phosphate groups that are present in nucleotides.

Front 32

What is the phosphate configuration found in DNA and RNA?

Back 32

C-5'

Front 33

In DNA, the Purines are ___&___ and the Pyridines are ___&____.

Back 33

PURINE:A&G
PYRIDINE:C&T

Front 34

In RNA, the Purines are ___&___ and the Pyridines are ___&____.

Back 34

PURINE:A&G
PYRIDINE:C&U

Front 35

_____ bonds are involved in DNA base pairings.

Back 35

Hyrdrogen

Front 36

Phosphodiester

Back 36

Bond between 3'C and 5'C

Front 37

At neutral salt, pH, and temperature, two complementary strands of DNA will spontaneously form a double helix.

Back 37

Annealing

Front 38

High heat or a strong basic solution will disrupt Hydrogen bonds and the strands will separate from each other.

Back 38

Denaturation

Front 39

When the DNA is in solution in test tubes

Back 39

Solution Hybridization

Front 40

When the DNA is bound to special types of filter paper.

Back 40

Filter Hybridization

Front 41

When the DNA is still within intact cells and/or chromosomes.

Back 41

In situ Hybridization

Front 42

Can denaturation process be reversed?

Back 42

YES

Front 43

The _____ molecules migrate at a faster rate through the gel than the _____ molecules.

Back 43

Smaller
Larger