Genetica Work Sheet Unit 3

Front 1

bacrerial growth lowest platou

Back 1

lag phase

Front 2

bacterial growth

increase

Back 2

log phase (growth)

Front 3

bacterai growth top platou

Back 3

stationary phase

Front 4

bacterial growth down

Back 4

death phase

Front 5

transformation

TF

Back 5

sghdjd

Front 6

Transduction

TD

Back 6

afvh

Front 7

colony

Back 7

visual cluster lf clones

~1 million cells

Front 8

lawn

Back 8

whole plate covered

Front 9

minimal media

complete media

Back 9

essentials

sugar (carbon source) + salts

bacteria synthesize aa, nucleotides, vitamins

as opposed to complete media

Front 10

selective media

Back 10

allows one species to grow and select against another

Front 11

prototroph

Back 11

organism synthesizes requirements from minimal media

Front 12

auxotroph

Back 12

nutritional mutant

requires one or more supplements to grow

ex. amino acids

Front 13

amp resistant

sensitivoty to strepromycin

auxotroph mutant requires trytophan

wild type

Back 13

Amp^r

Str^s

Trp^-

+

Front 14

macterial mutants

nutritional mutants

conditional mutants

resistant mutant

Back 14

auxotroph requires suplement to grow

mutation expressed in certain conditions

temp sensitive (ts)

antobiotic resistant

Front 15

bacteria dif colony and lawn

Back 15

one cell

million cloned cells

no isolated colonies whole plate

Front 16

conditional mutant and

nutritional mutant

Back 16

mutation related to condition (temp)

related to nutrients needed

Front 17

prototroph

and an

auxotroph

Back 17

naj

Front 18

binary fisision

and parasexual mating

Back 18

sggs

Front 19

plasmid and chromosome

Back 19

ssghs

Front 20

transduction and transformation

Back 20

svvs

Front 21

phage and a bacteria

Back 21

virus (can infect bacteria)

infect bacteria)

ct bacteria)

and living bacteria

Front 22

F+ cell

and

F- cell

Back 22

fsfa

Front 23

size of plasmid and size of E. coli genome

Back 23

scvs

Front 24

a non living plasmid

living bacteria

Back 24

vava

Front 25

lytic and lysogenic cycle

Back 25

agha

Front 26

virolent and a temperate phage

Back 26

vsbs

Front 27

phage absorption and

phage packing

Back 27

ndne

Front 28

operion inducer and repressor

Back 28

nenms

Front 29

Betagalactosidase and Z structural gene

Back 29

adfg

Front 30

promoter and operator

Back 30

dgvj

Front 31

I and l- O and O^c

Back 31

sgvvhj

Front 32

Lactose and glucose

Back 32

shgfjg

Front 33

polycistronic and monocistronic mRNA

Back 33

fyuhh

Front 34

constitutively expressed and regulated genes

Back 34

dhvbj

Front 35

bla gene

Back 35

a

Front 36

ori

Back 36

a

Front 37

GFP gene

Back 37

sfhsj

Front 38

restriction enzymes

Back 38

gshs

Front 39

lactose

Back 39

banw

Front 40

repressor protein

Back 40

f

Front 41

promoter

Back 41

b

Front 42

operator

Back 42

nxjdi

Front 43

lac Z

Back 43

e

Front 44

beta galactosidase

Back 44

sbsnks

Front 45

F Factor (plasmid)

Back 45

bs

Front 46

generalized transduction

Back 46

sbjsks

Front 47

adsorptions

Back 47

eejeoe

Front 48

extrachromosomally

episomally

Back 48

hejei

Front 49

F-

minus

Back 49

gzjbajs

Front 50

phage packing

Back 50

brjri

Front 51

F+ cell

Back 51

hsjs

Front 52

lysogeny

Back 52

bsbdi

Front 53

lysis

Back 53

bsnsjs