Genetic Engineering

Front 1

Selective Breeding

Back 1

A process farmers perform to allow successful individuals to reproduce.

Front 2

recombinant DNA

Back 2

DNA made from two or more different organisms.

Front 3

restriction enzymes

Back 3

bacterial enzymes that recognize and bind to a specific site on DNA and then cut the DNA between specific nucleotides.

Front 4

Vector

Back 4

An agent used to carry the gene of interest into another cell.

Front 5

Plasmid

Back 5

Circular DNA of bacteria.

Front 6

Gel electrophoresis

Back 6

A technique that uses an electric field within a gel to separate molecules by their size. The largest pieces of DNA are closest to the negative end of the gel.

Front 7

DNA fingerprint

Back 7

A pattern of dark bands unique to each person. Used in paternity and criminal cases.

Front 8

Transgenic organisms

Back 8

Organisms that have foreign DNA in their cells.

Front 9

Process of cloning

Back 9

1. Remove an egg cell from a sheep and take out the nucleus.
2. Remove a somatic (2n) cell from the organism to be cloned. Take out the nucleus.
3. Insert the somatic cell into the empty egg cell.
4. Implant the new egg cell into a surrogate mother to develop.

Front 10

Sticky ends

Back 10

Single stranded DNA that has been cut by restriction enzymes.

Front 11

Polymerase Chain Reaction )PR)

Back 11

A reaction to replicate the desired DNA to be run through gel electrophoresis.