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18 Cards in this Set
- Front
- Back
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what are the general requirements for specimen collection and transport?
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-Prior to antimicrobial drugs
-Avoid normal flora -Acquire during acute phase -Get enough -Use proper collecting device -Deliver to lab right away -get enough background info |
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What are the requirements for swab types?
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Use one of three types:
-Dacron/polyester -Cotton -Calcium alginate |
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What is safest swab type? Why?
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Dacron/polyester
-Cotton can have toxic fatty aa -Calcium alginate kills herpes |
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3 types of holding media are and contain:
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1. Sterile saline - non nutrient
2. Stuart's - buffered semi-solid 3. Amies media - charcoal |
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What is the purpose of charcoal in holding media?
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it absorbs toxins to keep fastidious neisseria gonorrhoea alive.
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What are 2 types of special transport media, and for what?
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1. Jembec - for gonorrhoeae
2. Regan lowe - for bordatella pertussis nasopharyngeal swabs. |
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What does a jembec plate have?
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-Modified thayer martin
-CO2 tablet for enriched, capnophilic environment. |
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When are swabs NOT preferred for specimen collection?
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-For anaerobic bacteria
-For UTI/stool specimens. |
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What are 2 sterile container types?
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1. Stuart's holding media
2. Cary-Blair media |
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What is Cary-Blair used for?
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Holding/preserving a stool specimen to find bacteria when immediate culture not possible.
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4 types of direct smear stains:
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-Gram
-Acridine orange -Methylene blue -Acid fast |
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What 2 things are noted when evaluating direct smears?
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-Number of bacteria
-Significance of host cells |
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What is a variation gram stain, and why do it?
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-Methanol fixation - overlay slide with methanol to fix.
-To lyse RBCs and decrease background. |
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What specimen is acridine orange used on?
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Blood cultures
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What is methylene blue used for?
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Fecal leukocytes
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Which prepared media are NOT exempt from quality control?
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-CA
-Campy BA -Selective neisseria agars (TM, MTM, Lewis) |
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What QC must be done on media prepared in house?
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-Sterility check
-Ability to support growth -Ability to be selective -Ability to give differential reactions |
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Other than media, what else is QC run on?
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-Reagents/antisera
-Kits -Antimicrobial suscept. testing |
