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12 Cards in this Set
- Front
- Back
used to lacalize the Huntington's gene relative to other genetic markers
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genetic map
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a gene that has been mapped previously
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genetic marker
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the difference between the presence and absence of certain restriction enzyme sites
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restriction fragment length polymorphisms
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procedure that uses probes to identify the polymorphisms between two people - but each individual is marked individually and u just make the comparison yourself
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southern blot
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PCRs role in genetic marking of huntington disease (or any other disease)
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PCR makes copies of the gene and amplifies the gene so that we could see the number of repeats of a specific codon
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germ-line transformation
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introducing a new gene (transgene) into a fertilized egg, which means that every daughter cell will be affected and even for generations after that
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somatic cell transformation
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introducing a transgene into a regular cell like a blood, bone, intestinal cell so that it affects that cell itself but is not passe down to daughter cells
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adenovirus
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viruses that are safe bc they go into the genome of the host clel but they do not incorporate their own genes into the cell
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ti plasmid
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this is used in agrobacterium tumefaciens, for the transformation of plants
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genetic engineering of plants
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use a t-plasmid
replace the tumor gene with a desired gene and incorporate it into the cells |
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what must an engineered t-plasmid have?!?!?
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promoter!!!!!
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genomics
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the effort to sequence, interpret, and compare whole genomes or various, bacteria and archaea
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