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19 Cards in this Set
- Front
- Back
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Genetic engineering |
Alteration of an organism's genome |
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Recombinant DNA Technology |
Collection of methods used to create DNA molecules by in vitro ligation of DNA from 2 different organisms, and replication and recovery of such recombinant DNA molecules |
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Biotechnology |
Use of living organisms to create a product or process that helps improve the quality of life for humans or other organisms |
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Plasmids |
Extrachromosomal double-stranded DNA molecules that replicate independently from the chromosomes within bacterial cells |
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Restriction enzyme |
enzyme produced chiefly by certain bacteria, having the property of cleaving DNA molecules at or near a specific sequence of bases |
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Restriction site |
Sites located on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides, which are recognized by restriction enzymes |
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Rhizobium radiobacter |
-causal agent of crown gall disease in over 140 species of eudicots - rod-shaped, Gram-negative soil bacterium |
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TDNA |
Transferred DNA of the tumor-inducing (Ti) plasmid of some species of bacteria |
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Bacillus thurgiensis |
Bacterium with gene used to encode proteins that kills insects and larvae in Bt corn |
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Daffodil and bacteria |
Sources of genes uses to enhance levels of B-carotenoid in golden rice |
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Gene therapy |
experimental technique that uses genes to treat or prevent disease. In the future, this technique may allow doctors to treat a disorder by inserting a gene into a patient's cells |
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1. Cloned human ADA gene is transfered to viral vector, then used to infect white blood cells removed from patient 2. Gene is incorporated to chromosome 3. After growth to enhance numbers, cells are inserted back to human 4. Immune system develops |
Ashanti deSilva treatment |
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Clustered regularly-interspaced short palindromic repeats |
CRISPR |
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Cas9, guide RNA |
Components of CRISPR |
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Genome editing |
Group of technologies that allows scientists the ability to change an organism's DNA |
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CRISPR arrays |
CRISPR DNA segments created using snippets of invading viruses |
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Polymerase chain reaction |
Amplifies small segments of DNA used in fingerprinting etc. |
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Nucleus of unfertilized egg or zygote is replaced eith nucleus of differentiated cell |
Nuclear transplantation |
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1. Nucleus from mammary cell is transplanted into egg cell wih no nucleus 2. Fused cell is grown in culture 3. Early embryo is implanted in another sheep 4. Embyonic development |
Reproductive sheep cloning |
