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8 Cards in this Set
- Front
- Back
What is the purpose of gas chromatography? |
To separate and analyse components of a mixture on the basis of boiling point (volatility). |
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What happens to molecules in a mixture of substances when a sample is injected into a GC instrument? |
Converts into gas, molecules vaporised and carried by inert gas into column. |
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What happens to the molecules in a mixture of substances after the injection of the sample? |
Molecules travel through column and mixture separates into individual components on the basis of different affinity of molecules with stationary phase. |
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What happens to sample molecules as they are introduced into a mass spectrometer? |
Molecules are blasted with electrons which cause them to break down into positively charged fragments. |
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What do the signals in a mass spectrum represent? |
Mass of fragmented ions. |
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How can unknown compounds be identified using MS? |
Similarity search database |
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Explain what GCMS is being used to determine in this demonstration? |
Testing purity of aspirin, monitor reaction progress and outcome of reactions. |
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Describe other applications of GCMS |
-Toxicity analysis of food or environmental samples |