Gapcom 4/12 Bacterial Morphology And Cell Structure (X)

Front 1

differences btw eukaryotic and prokaryotic cells that can be used as targets by drugs

Back 1

1. dif ribosomes (70S)
2. cell wall/peptidoglycan
3. flagellum

Front 2

steps to do gram stain. what will it differentiate.

Back 2

crystal violet, gram's iodine, decolorizer (alochol or acetone), safranin red. purple indicates gram positive. red indicates gram negative.

Front 3

list structures of bacterial cells

Back 3

chromosome (single dsDNA circular), plasmids, ribosomes, cytoplasmic membrane (lipid bilayer, e- transport occurs across), cell wall (gm +/-), capsule (thick polysaccharide for adherence; also called slime layer/glycocalyx), flagella (motility), fimbriae or pili (attachment struct), sex pili

Front 4

what are the morphologic dif btw gram positive and negative cells?

Back 4

gm-positive: thick peptidoglycan, No LPS, contains teichoic acids, senstive to lysozyme, more sensitive to penicillin (bc more peptidoglycan), some have spores and capsules.
gm-negative: thin peptidoglycan, contains LPS, No Teichoic acids, resistant to lysozyme, more resistant to penicillin, no spores, some have capsules.

Front 5

explain the capsule in the bacterial cell structure.

Back 5

outermost struct of bacteria. capsule is tightly bound to cell wall. slime is loosely adherent to cell wall. usu polysaccharide. main fxn: prevent phagocytosis. barrier to toxic compounds, & promotes adherence. Ab to capsule useful in serologic diagnosis and vaccine production.

Front 6

describe char of spores. give ex. of bacteria that produce them.

Back 6

form under harsh conditions to survive for long periods. complete chrom with essential proteins, ribosomes, and 2 peptidoglycan layers, with outer protein coat. refract light. vegtative cell growth stage. only in gm-positive bacteria (ex. bacillus & clostridium)