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6 Cards in this Set

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differences btw eukaryotic and prokaryotic cells that can be used as targets by drugs
1. dif ribosomes (70S)
2. cell wall/peptidoglycan
3. flagellum
steps to do gram stain. what will it differentiate.
crystal violet, gram's iodine, decolorizer (alochol or acetone), safranin red. purple indicates gram positive. red indicates gram negative.
list structures of bacterial cells
chromosome (single dsDNA circular), plasmids, ribosomes, cytoplasmic membrane (lipid bilayer, e- transport occurs across), cell wall (gm +/-), capsule (thick polysaccharide for adherence; also called slime layer/glycocalyx), flagella (motility), fimbriae or pili (attachment struct), sex pili
what are the morphologic dif btw gram positive and negative cells?
gm-positive: thick peptidoglycan, No LPS, contains teichoic acids, senstive to lysozyme, more sensitive to penicillin (bc more peptidoglycan), some have spores and capsules.
gm-negative: thin peptidoglycan, contains LPS, No Teichoic acids, resistant to lysozyme, more resistant to penicillin, no spores, some have capsules.
explain the capsule in the bacterial cell structure.
outermost struct of bacteria. capsule is tightly bound to cell wall. slime is loosely adherent to cell wall. usu polysaccharide. main fxn: prevent phagocytosis. barrier to toxic compounds, & promotes adherence. Ab to capsule useful in serologic diagnosis and vaccine production.
describe char of spores. give ex. of bacteria that produce them.
form under harsh conditions to survive for long periods. complete chrom with essential proteins, ribosomes, and 2 peptidoglycan layers, with outer protein coat. refract light. vegtative cell growth stage. only in gm-positive bacteria (ex. bacillus & clostridium)