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9 Cards in this Set
- Front
- Back
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Use frozen sections for |
Rapid H&E Enzyme histochemistry Immunofluorescence Immunohistochemistry Other stains |
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Frozen sections are done on what type of microtome? |
Cryostat |
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Frozen sections use what kind of tissue? |
Fresh tissue |
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Most tissues should be cut at what temp? |
-20° C |
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Breast tissue should be cut at what temp? |
-25° C |
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Brain, liver, spleen & lymph node should be cut at what temp? |
-10° C |
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Steps for cutting with a cryostat (4) |
1) cryoembed specimen 2) rapidly freeze specimen 3) roughcut/ face in 4) obtain section directly on slide |
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What type of artifact is most common with frozen sections? |
Freeze thaw artifact |
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What are the 2 ways to to prevent rolling sections? |
Antiroll plate Brush technique |
