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58 Cards in this Set
- Front
- Back
Who was L. M. named after
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Lord Lister
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what are some of the reasons for the emergence of Listeria monocyogenes? (6)
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improved medical diagnostics
improved isolation and detection strategies increased number of immunocompromised and the elderly Food production changes -cold storage and extend shelf lives Popularity of cooked and ready to eat foods serious manifestation of illness |
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Where and what was the first major outbreak of lm
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a california outbreak from mexican style cheese --- raw milk in it
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What is the fatality rate of lm
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20-30%
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T of F. lm has an ubiquitous nature ie it can be found anywhere
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true
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Is lm psychrotrophic
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yes, it will grow in a fridge
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at what acid level can lm survive
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4.6
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lm has been found in up to ___% brine solutions
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22
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The united states has a ____ tolerance level for lm
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zero
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how many species of lm are there currently
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6
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the ____ strain in the only public health concern
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listeria monocytogenese
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Cold enrichment isolation can take ____ to _____
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months
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What are the three steps to USDA lm isolation?
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primary enrichment
secondary enrichment plate: modified oxford agar |
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Is lm gram + or gram -
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gram +
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Does it have a long or short rod
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short
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does it come in singles or pairs
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both
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is it a spore or non spore former
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non spore former
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What does peritrichous flagella mean
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it means an lm cell has flagella all around it
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at what degree temperature does lm have tumbling motility
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20-25 degrees, not at 37
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what is the growth range of temperature, pH and salt for lm
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0-45 degree c, 4.4-9.6 and <10% salt
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At what temperature and for how long does milk need to be heated for to kill lm
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161 for 15 seconds
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what temp must meat be cooked to to kill lm
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165
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T or F there is an increased thermal tolerancer following heat shock and stress adaptation
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true
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Does lm survive freezing or not
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yes
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does lm grow at pH < 4.3
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no!!!!!!!!
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Low or high storage temperatures favor survival
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low
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can lm adapt to acid
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yes
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what is the cut off point for Aw where lm can no longer grow
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<.93
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Product preservation can occur with things like sodium diacetate, propionic acid, sorbic acid, benzoic acid, sodium nitrite, liquid smoke, spices etc
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fact
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What are the the major antibiotic-like proteins that can be added to products to preserve them
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NISIN and pediocin
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combined treatments create the ____ concept
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hurdle
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UV irradiation if only good for ____
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surface cleaning
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High ______ pressure is another way of ridding LM
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hydrostatic
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t or f. lm is not found in soil, rivers, and silage
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false. it is also found in lakes, sewage, pasture grass
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lm can enter a food processing plant on soil of shoes, clothing, raw product and human carriers
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fact
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Can lm gather in floor drains, condensation, coolers, aerosols and equipment residue
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uh duh, yeah
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Up to ___% of carcasses in slaughterhouses are infected
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50
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t or f 23% of refrigerator foods from listeriosis victims are positive
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false, it's 11%
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___ to ___ % of raw milk has lm
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2-5
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t or f. cheese is a great source of lm
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TRUE, like brie and caminbare
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__ to ___% is found in cheese
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2-10
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___ to ____% found in ice cream
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.3-2
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how frequent is lm in yogurt and butter
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not frequently at all
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a major source of lm is ____, with a growth of 10^6 CFU/g
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pate
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___ to _____ % of lm found in poultry
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0-60
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__ to ___ % lm found in liquid egg
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0-15
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is lm found in low or high numbers in things like fresh veggies, tomatoes and carrot
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low
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problem products in the fish and seafood area are ___ ____ and ____
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smoked fish, cooked crab and smoked mussells
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2-10% of humans are asymptomatic fecal shedders
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fact
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listeriosis patiencts frequently excrete how much Lm
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> 10^4 CFU/g
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an outbreak can occur if there is > ___ CFU/g
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100
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one of the main problems of lm is the long incubation time, which is _______
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3-70 days
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What are the susceptible populations
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neonates, elderly, pregnant women, immunocompromised, those with chronic diseases, but is CAN affect normal, health people as well
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what is the incidence of lm
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2-7 cases/1million people
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how many cases of lm are there in the united states each year
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1200-1600
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most cases are sporadic or nosocomial, which means what
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infections that are acquired in the hospital
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if pregnant women come down with lm the fetus can spontaneously ____
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die
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Mexican style cheese in Los Angelos county put LM _____
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on the map
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