Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
17 Cards in this Set
- Front
- Back
|
What are the two methods to measure glucose in plasma or urine?
|
Alkaline copper reagent (positive result with any reducing compound)
Glucose oxidase (enzyme) (specific for glucose) |
|
|
Enzymes are measured by their mass and molarity T/F
|
FALSE they are measured by their CATALYTIC activity
|
|
|
What are the standard units of enzyme activity?
|
1 μmol of product formed/min at 30*C
|
|
|
Give three phsysiological ways enzyme activity is regulated in the body
|
Change in rate of synthesis of the enzyme (slow)
Covalent modification of the enzyme (fast) Activation by precursors or inhibition by end-products (instantaneous) |
|
|
Reversible inhibitors bind to the enzyme OR enzyme-substrate complex *covalently* T/F
|
F, non-covalently
|
|
|
Most reversible inhibitors are highly specific T/F
|
FALSE, relatively unspecific (will inhibit a number of enzymes)
|
|
|
Most reversible inhibitors are relatively unspecific T/F
|
T
|
|
|
Reversible inhibitors diffuse onto and off the enzyme, and therefore undergo metabolism and excretion. What is a consequence of this? (Drug wise)
|
Doses are required relatively frequently
|
|
|
What are the three main types of reversible inhibition?
|
Competitive
Non-competitive Uncompetitive |
|
|
A competitive inhibitor forms an enzyme-inhibitor complex T/F
|
T (i.e. it competes with the substrate for the formation of the enzyme-substrate complex
|
|
|
A compeitive inhibitor competes with the substrate for the formation of an ESC T/F
|
T
|
|
|
A competitive inhibitor decreases V(max) T/F
|
F, V(max) stays the same
|
|
|
A competitive inhibitor increases the apparent K(m) T/F
|
T
|
|
|
This type of inhibition proceeds to slow formation of the product (i.e. it reduces the rate of reaction at all concentrations of the substrate by interfering with the catalytic mechanism)
|
Non-competitive inhibition
|
|
|
A non-competitive inhibitor has no effect on the apparent K(m) T/F
|
T
|
|
|
A non-compeitive inhibitor increases V(max) T/F
|
F (It decreases)
|
|
|
An uncompetitive inhibitor reduces K(m) and V(max) T/F
|
T
|
