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5 Cards in this Set
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Steps for using a fluorescence microsocpe |
1. koehler illumination maximum resolution optical centering diopter correction 2. establish phase contrast and adjust focal plane 3. start burner for fluorescent lamp 4. set cube turret 5. to control fluorescence illumination(pull/push shutter) 6. set beam splitter to mid point (sends light to both oculars and camera). set camera only for very low emission intensities |
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WIB |
wide interference blue (use for fluorescein; FITC; DiOC6; acridine orange) |
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WIY |
wide interference yellow-green (use for rhodamine; TRITC) |
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WU |
wide interference ultraviolet (use for DAPI; Hoechst) |
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O |
open |
