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32 Cards in this Set
- Front
- Back
Phenylalanine
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POS: Proteus, mirabilis, providencia
neg: everything else |
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Test after phenylalanine; what it shows:
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Indole: shows
The only neg is -> P. mirabilis Pos -> Do an ornithine |
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Ornithine results of phenyl/indole POS
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Ornithine pos: Morganella
Ornithine neg: do an H2S to differentiate P. vulgaris and Providen. |
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differentiation of P. vulgaris and Providencia:
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H2S:
pos = p. vulgaris neg = providencia |
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What test is done on Phe Neg bugs
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Voges Proskauer
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VP results:
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POS = klebsielliae tribe
Klebsiella, Enterobacter, Serratia Neg = SEE the SCY Salmonella, E. coli, Edwardsiella, Shigella, Citrobacter, Y. enterocolitica |
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1st test to run on VP pos organisms
Results? |
Indole;
Pos: Klebsiella oxytoca Neg: all else; do a Lys/Ornithine |
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On VP pos/Indole neg bugs, what test to run, what is the result?
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Lysine/Ornithine.
Lys+/Orni- -> Klebsiella pneumoniae. Any other combo -> others -> do DNAse |
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What test is done on VP pos after lys/ornithine on "all else"?
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DNAse or Gelatin
DNAse pos = Serratia Dnase neg = Enterobacter |
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What organisms are Phe neg, VP neg?
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See the Scy
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First test to run on Phe neg, VP neg:
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Lysine
Pos = SEE; Salmonella, Edwardsiella, E. coli Neg = SCY Shigella, Citrobacter, Yersinia |
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1st test on Phe +, VP -, Lys pos:
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H2S;
Only neg = E. coli Pos = do an INdole |
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Indole result of Phe pos, VP neg, LYS pos, H2S pos:
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Differentiates Edwardsiella/Salmonella:
Indole Pos = Edwardsiella Neg = Salmonella |
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First test to run on Phe neg, VP neg, LYS neg:
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H2S:
Pos: C. freundii Neg: do a Citrate |
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what test is run on Phe neg, VP neg, Lys neg, H2S neg?
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Citrate;
Pos = Citrobacter koseri Neg = Yersinia/Shigella - do a TSI |
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Last test to run on Phe neg, VP neg, Lys neg, H2S neg, Citrate neg:
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TSI:
Pos = Yersinia enterocolitica Neg = Shigella YES! |
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If it's citrate positive, isolate cannot be:
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SEE MY
Shigella, Edwardsiella, E. coli Morganella, Yersinia enterocolitica |
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If it's H2S positive, isolate can only be
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SPEC
Salmonella Proteus Edwardsiella Citrobacter freundii |
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If Urea is 4+ isolate can only be:
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Proteus, Morganella, some Providencia
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If TSI is A/A(g) isolate cannot be:
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shigella, edwardsiella, Proteus mirabilis, Morganella, Providencia
These are always K/A only glucose!!! |
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the isolates that are NEVER A/A are:
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PEMPS
Edwardsiella, Morganella, P. mirabilis, Providencia, Shigella |
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If lysine is deaminated, the isolate can only be:
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Proteus, morganella, or providencia
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If VP is positive, isolate can only be
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Klebsiella oxytoca/pneumonia
Enterobacter Serratia 50% of Proteus mirabilis |
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If motility is pos at 37 degrees C, isolate cannot be:
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Shigella, Klebsiella, or Yersinia
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Of the klebsiellas, which is indole pos/neg?
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K. oxytoca: Indole pos
K. pneumoniae: neg |
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Of the enterobacters, which are ornithine pos?
neg? |
E. cloacae
E. aerogenes Neg = E. agglomerans |
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How to tell Enterobacter agglomerans from other species:
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Neg on Ornithine, lysine, and arginine
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How to tell Enterobacter cloacae from aerogenes and agglomerans:
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+ornithine, +arg, -neg lysine
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How to tell Enterobacter aerogenes from Enterobacter cloacae and Enterobacter agglomerans:
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+Pos ornithine, -Neg arginine, Pos lys
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How to speciate Enterobacter:
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Orn Arg Lys
Cloacae + + - Aerogenes + - + Agglomerans - - - |
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How to speciate Citrobacter:
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H2S Indole
C. freundii + - C. koseri - + |
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How to speciate Proteus:
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Indole Ornithine
Mirabilis - + Vulgaris + - |