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26 Cards in this Set
- Front
- Back
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10% Formalin
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-1 part 37-40% formaldehyde, 9 parts water
-contains 3.7-4% formaldehyde |
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10% Neutral buffered formalin
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-10% formalin, sodium phosphate monobasic & sodium phosphate dibasic
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Zenker solution
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-potassium dichromate, mercuric chloride, distilled water, & glacial acetic acid
-recommended for bone marrow, blood forming organs, connective tissue and muscle, PTAH stain, Giemsa, & mucin |
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B-5
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-mercuric chloride, sodium acetate, distilled water, & formaldehyde
-recommended for bone marrow |
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Bouin solution
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-aqueous picric acid, formaldehyde, acetic acid
-recommended for embryos, trichrome stain, skin, hard fibrous tissue -decals as it fixes -good nuclear fixative -hemolysis and does not fix glycogen |
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Helly solution
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-mercuric chloride, potassium dichormate, distilled water, & formaldehyde
-recommended for pancreas, bone marrow, blood containing organs, & pituitary |
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Gendre solution
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-95% alcohol saturated with picric acid, formaldehyde, acetic acid
-recommended for glycogen & polysaccharides |
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Hollande solution
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-copper acetate, picric acid, formaldehyde, acetic acid, distilled water
-good fixative for GI biopsy specimens -also decals small bone specimens |
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Orth solution
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-distilled water, formaldehyde, potassium dichromate, sodium sulfate
-recommended for glycogen, adipose, & especially chromaffin cells |
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Zamboni solution
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-picric acid, paraformaldehyde, sodium phosphate monobasic & dibasic
-recommended for electron microscopy -stabilizes cellular protein - aka buffered picric acid-formaldehyde (PAF) |
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Carnoy solution
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-chloroform, glacial acetic acid, ethyl alcohol
-recommended for glycogen, Nissl substances (granules in the neurons), chromosomes, muscles -causes shrinkage and hemolysis |
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Zinc formalin
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-fixes faster than formalin
-zinc has been reported to undo some of the deleterious effects of prior formalin fixation -gives better results IHC than NBF |
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Potassium dichromate
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-non-coagulant if unacidified, coagulant if acidified
-fixes mitochondria by rendering their lipid components insoluble in lipid solvents |
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mercuric chloride
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-poisonous
-acts as permanent mordant and produces brilliant staining -good fixative for mucin -zinc chloride is a good substitute |
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picric acid
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-both a fixative and a dye
-excellent coagulant of proteins -good for glycogen -highly explosive |
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formaldehyde
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-most widely used fixative agent
-if not buffered will become acidic -additive, non-coagulant |
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glutaraldehyde
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-Used as fixative for electron microscopy b/c it preserves ultrastructure.
-a dialdehyde -causes a positive PAS reaction and a positive GMS reaction |
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paraformaldehyde
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-highly polymeric form of formaldehyde
-commonly used for electron microscopy |
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osmium tetroxide
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A fixative that is mostly used in electron microscopy because it preserves lipids.
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Formalin ammonium bromide (FAM)
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-Recommended for tissue of the central nervous system
-slow fixation |
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Alcoholic formalin
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-consists of formaldehyde, absolute ethyl alcohol, distilled water
-recommended for glycogen |
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Acidic formalin
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-formaldehyde and acetic acid
-recommended for gold techniques and impregnation of glial elements -forms a formic acid pigment |
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Ethyl Alcohol
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-recommended for glycogen, blood smears, proteins and enzymes
-dehydrates & fixes at the same time -causes excessive shrinkage and hardening |
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Clark's solution
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-absolute alcohol, glacial acetic acid
-recommended for smear preparations of cell cultures |
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Fleming's
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-chromic acid, osmium tetroxide, glacial acetic acid
-recommended for cytoplasmic structures -fixes fat and give excellent nuclear detail -toxic and expensive |
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Vande Grift's
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-ethanol, formaldehyde, glacial acetic acid, picric acid, mercuric chloride
-recommended for urea |
