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20 Cards in this Set
- Front
- Back
Giemsa stain used for?
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Borrelia
Plasmodium trypanosomes Chlamydia |
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Culture requirement for H. influenza
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Chocolate agar with Factor V (NAD+) and Factor X (hematin)
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Culture requirement for N. gonorrhoea
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Thayer Martin or VPN (vanco - inhibits gram positives, polymyxin - inhibits gram negatives, nystatin - inhibits fungi)
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Culture requirement for B. pertussis
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Bordet-Gengou (potato) agar
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Culture requirement for C. diptheriae
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Tellurite plate (Loeffler's media
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culture requirement for M. tuberculosis
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Lowenstein-Jensen agar
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culture requirement for M. pneumoniae
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Eaton's agar
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culture requirement for lactose fermenters
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pink colonies on MacConkey's agar
E. coli can also be grown on eosin-methylene blue (EMB) agar as blue-green colonies with metallic sheen |
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culture requirement for Legionella
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Charcoal yeast extract agar with iron and cysteine
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culture requirement for fungi
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Sabouraud's agar
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Obligate aerobes
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Nocardia, Pseudomonas aeruginosa, Mycobacterium tuberculosis, and Bacillus
Nagging Pests Must Breathe |
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Obligate anaerobes
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Clostridium, Bactereoides, Actinomyces
•AminOglycosides are useless here because they need O2 to get into cell |
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encapsulated bacteria
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Strep Pneumo
H. influenza - type b N. meningitidis Salmonella GBS Klebsialla |
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urease positive bugs
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Klebsialla
Proteus Ureaplasma Nocardia Cryptococcus H. pylori |
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what is a superantigen and which bacteria have them?
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binds directly to MHC II and TCR to activate large numbers of T cells and stimulate release of gamma interferon and IL-2
S. aureus - TSST-1 causes toxic shock syndrome (fever, rash, shock) S. pyogenes - erythrogenic toxin causes toxic shock-like syndrome |
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which bacteria have ADP ribosylating toxins?
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Corynebacterium - inactivates EF2 --> causes pharyngitis and pseudomembranes in throat
Vibrio cholera - activates Gs --> pumping of Cl- into gut and decreasing Na+ reabsorption --> rice-water stool E. coli - LT stimulates cAMP and ST activates cGMP --> watery diarrhea Bordatella pertussis - inhibits Gi --> also leading to increased cAMP --> whooping cough |
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Clostridium perfringens
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alpha toxin - lecithinase that cleaves membranes --> myonecrosis and gas gangrene
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C. tetani - toxin
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blocks release of GABA and glycine from Renshaw inhobitory interneurons in the spinal cord
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C. butulinum toxin
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blocks release of Ach at NMJ
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B. anthracis - toxin
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edema factor, lethal factor and protective facrtor
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