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20 Cards in this Set

  • Front
  • Back
Giemsa stain used for?
Borrelia
Plasmodium
trypanosomes
Chlamydia
Culture requirement for H. influenza
Chocolate agar with Factor V (NAD+) and Factor X (hematin)
Culture requirement for N. gonorrhoea
Thayer Martin or VPN (vanco - inhibits gram positives, polymyxin - inhibits gram negatives, nystatin - inhibits fungi)
Culture requirement for B. pertussis
Bordet-Gengou (potato) agar
Culture requirement for C. diptheriae
Tellurite plate (Loeffler's media
culture requirement for M. tuberculosis
Lowenstein-Jensen agar
culture requirement for M. pneumoniae
Eaton's agar
culture requirement for lactose fermenters
pink colonies on MacConkey's agar

E. coli can also be grown on eosin-methylene blue (EMB) agar as blue-green colonies with metallic sheen
culture requirement for Legionella
Charcoal yeast extract agar with iron and cysteine
culture requirement for fungi
Sabouraud's agar
Obligate aerobes
Nocardia, Pseudomonas aeruginosa, Mycobacterium tuberculosis, and Bacillus

Nagging Pests Must Breathe
Obligate anaerobes
Clostridium, Bactereoides, Actinomyces

•AminOglycosides are useless here because they need O2 to get into cell
encapsulated bacteria
Strep Pneumo
H. influenza - type b
N. meningitidis
Salmonella
GBS
Klebsialla
urease positive bugs
Klebsialla
Proteus
Ureaplasma
Nocardia
Cryptococcus
H. pylori
what is a superantigen and which bacteria have them?
binds directly to MHC II and TCR to activate large numbers of T cells and stimulate release of gamma interferon and IL-2

S. aureus - TSST-1 causes toxic shock syndrome (fever, rash, shock)

S. pyogenes - erythrogenic toxin causes toxic shock-like syndrome
which bacteria have ADP ribosylating toxins?
Corynebacterium - inactivates EF2 --> causes pharyngitis and pseudomembranes in throat

Vibrio cholera - activates Gs --> pumping of Cl- into gut and decreasing Na+ reabsorption --> rice-water stool

E. coli - LT stimulates cAMP and ST activates cGMP --> watery diarrhea

Bordatella pertussis - inhibits Gi --> also leading to increased cAMP --> whooping cough
Clostridium perfringens
alpha toxin - lecithinase that cleaves membranes --> myonecrosis and gas gangrene
C. tetani - toxin
blocks release of GABA and glycine from Renshaw inhobitory interneurons in the spinal cord
C. butulinum toxin
blocks release of Ach at NMJ
B. anthracis - toxin
edema factor, lethal factor and protective facrtor