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35 Cards in this Set

  • Front
  • Back
What is a liquid Sterilant
Glutaraldehyde
What is a narrow spectrum antibiotic against gram positive bacteria; inhibits PG crosslinking
Penicillin
What is an effective antibiotic for Pseudomonas
gentomycin
An antibiotic that mimics PABA

Sulfa drugs



Is Tetracycline a narrow spectrum antibiotic?
No it is a broad one
Give an example of a disinfectant from which works with organic gram positive bacteria.
Quats
Is Mycobacterium an acid fast bacteria and what kind of disinfectant is effective against it?

Yes, Phenolic compounds and Aldehydes are effective



Can Hydrogen peroxide inhibit Endospore, acid fast bacteria and Prions?
Yes

Name 3 disinfectants/antiseptic that denature portents and dissolve lipids?
Alcohols, isopropyl, and ethanol
Iodine and Chlorine are examples of what protein oxidizing agents?
Halogens

Do heavy metals deoxidise and denature proteins?
yes
What is FAIRLY effective against Endospore?

Peroxyns



What are effective gas disinfectants?

Chlorine and ethylene



Name three antibiotics which inhibit cell wall synthesis
Penicillin, cephalosporin, and vancomycin
Name two antibiotics that inhibits protein synthesis
chloramphenicol, and tetracyclines
What are 3 antibiotics that interfere with plasma membranes?
Neomycin, nystanin, and polymyxin B
Do Rifamycin and quinolone interfere with DNA synthesis, RNA and enzyme function?

Yes



What are surfactants and do they disinfect?
Its soap and no it does not disinfect. In fact it has very little effect.
In a last resort case what health care people give you for an antibiotic?
Vancomycin
Is PEA used for Gram Negative bacteria?
No it is used for gram positive bacteria
What broth is used for anaerobic bacteria?
Thiohlycolate
Media that can be used as differential media but can also be used for gram positive bacteria.
BAP through the use of hemolysis
Can solid media be used to differentiate bacteria motility?
No it has be to semi-solid

True/False


TSI can be used to distinguish the production of H2S (red) and acid production.

False. The slant turns black and yellow not red and yellow
What type of test is used to distinguish between streptococci and staphylococci?
Catalase. It bubbles if positive

True/False


Oxidase turns purple when in the presence of gram positive bacteria.

False it turns purple in the presence of gram negative bacteria
What are the colours used to differentiate hemolysis?

B-clear


A-green


Y-red

What color is a lactose carbohydrate broth incubating lactose fermenting bacteria?
yellow

what color is lactose fermenting bacteria on MAC and why is it that color?

It is pink because it is gram negative media



What are the phases of a growth graph?
Lag phase, exponential phase, stationary phase, and death phase.
What is the difference between psycotrophs and mesophiles?
The psycotrophs optimum growth is 20-30C and are normally seen in food spoilage. Mesophiles have a 25-40C and are seen in diseases.
Explain where Obligate anaerobes and aerobes are located in a broth.
Anaerobes are located at the top and need O2 and aerobes are located at the bottom and don't need O2.
Where are microearophiles located?
They are located in the middle if seen in a test tube.
How would you grow Microaerophiles?
You would use a candle jar.
Name the Macroelements.

Nitrogen


Sulfur


Phosphorous