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17 Cards in this Set
- Front
- Back
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Identify normal daily dietary fat intake
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60-100 g/day
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Explain what the maxiumum amount of fat that may be absorbed daily through the intestines and what happens to the excess that is not absorbed
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>150 will not be absorbed
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Define steatorrhea and describe its symptoms
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abnormal increase of fat in the stool characterized by big, pale, greasy, bulky, pasty, fatty, frothy, foamy, foul smelling, floating feces.
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Why does steatorrhea cause demineralization of the bone?
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Fat in the intestine will get turned into soap by means of calcium. Calcium from the diet cannot be absorbed because it is bound to fat and excreted in the feces. The body must get calcium from the bone in order to compensate.
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Identify the three classifications of steatorrhea
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Enterogenous
Hepatongenous Pancreatogenous |
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What are some of the various conditions related to Enterogenous steatorrhea
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Gluten induced (inflammation)
Tropical sprue Intestinal lymphoma Surgical loss of functional bowel Acute or chronic diarrhea syndromes Atrophy of malnutrition |
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What are the reagents necessary for a Qualitative exam for fecal fat.
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-95% ethanol
Sudan III or IV or Oil red-O 36% acetic acid Heat |
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Describe the gravimetric method for a quantitative examination for fecal fat.
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Weigh out 10 g of poop
Bake @ 350 to dry out Use a fat solble solvent to mix with stool then centrifuge Evaporate solvent Left with fat Weigh fat |
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State the principle of electrophoresis
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Charged particles or molecules when placed in an electrical field will migrate towards the opposite charge.
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What are seven factors that influence the rate of migration in an electrically chared feild
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Charge
Size and molecular weight Shape Strength of electrical field Tpe of support media pH Time |
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Recall the pH of the buffer used in most SPE procedures
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Barbital buffer (pH 8.0-9.0)
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What charge will a protein have if the pH <pI
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Protein will have a + charge
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What charge will a protein have if the pH >pI
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Protein will have a = charge
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What is the isoelectric point of albumin
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4.9
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What is the isoelectric point of gammaglobulins
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8.6
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Inflammation, malignancy, and kidney disease would cause and increase in _________and a decrease in _______
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Alpha globulins
Albumin |
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List some examples that would cause an increase in Beta globulins
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Pregnancy, HIgh cholesterol, Liver disease
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