Family Bacteroidaceae

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Family Bacteroidaceae

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Slow-growing, foul smelling, anaerobic, non-sporing

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obligate anaerobes, G -, non spore, straight curved helical rods. Mot +/-, chemoorganotrophic, metabolizing carbs, peptones, or metabolic intermediates

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Family BActeroidaceae

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Gram -, nonspore straight rods, mot +/-, peritrichious flagella.
Fermentation products: succinic, lactate, propionate, acetic, frmic acids
Opt temp 37 C, pH 7.o

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Bacteroides

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Isolation of Bacteroides

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Complex medium, peptone, YE, vit K, and hemin

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Which genera can transform bile acids in the gut?

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Bacteroides

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found in vagina 60%, nonhemolytic, grows on 20% bile, cat +, Survives in air 6 - 8 hrs, hemin stimulates growth

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BActeroides fragilis

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treatment of Bacteroides fragilis

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clindamycin

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Pathogenic factors of Bacteroides fragilis?

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capsule, surface hemagglutinin, superoxide dismutase

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Does not grow in 20% bile, hemin and menadione needed for growth, black colonies, fluoresce red under UV light

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BActeroides melaninogenicus

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Nonspore, G - rods, morphology varies, anerobic, mot -/+,
Fermentation products butyric, acetic, lactic acids

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Fusobacterium

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DNase+, grows in presence of 6% oxygen, nonhemolytic, bread crumb appearance on media

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Fusobacterium nucleatum

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G-, short rods, anaerobes, mot -

Ferm products butyric and acetic acid. Produces black pigment, fluoresces red

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Porphyromonas

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Name a species that belongs to the genera Porphyromonas

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Porphyromonas gingivalis

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G-, anaerobe, straight and curved rods, mot -, cat -, H2S-, NO3 red.-, opt. temp 35- 37C

Ferm 90% lactic and 10% acetic acids

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Leptotrichia

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Species that belongs to genus Leptotrichia

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Leptotrichia buccalis

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G-, curved rods, anaerobicm mot +, polar falgellum

Ferm butyric acid

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Butyrivibrio

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CCellulose digester in cattle, isolated using Rumen Fluid Agar

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Butyrivibiro fibrisolvens

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G-, anaerobic, helical, mot +, polar flagella, NO3 -, cat -, gel -,indole -, H2S -

Ferm Products succinic, acetic, fromic, lactic

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Succinivibrio

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Dextrin dissolving strain

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Succinivibrio dextrinsolvens

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G-, straight rods, anaerobes, mot +, polar flagella, cat -

Ferm succinic and acetic acids

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Succinomonas

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Starch dissolving, 6% of the rumen flora

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Succinomonas amylolytica

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G -, curved rods, mot +, lateral/polar, methanol production, indole -, H2S -, NO3-, gel-

Fermlactic, formic, acetic, ethanol, H2 gas, CO2

Pectin Fermented

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Lachnospira

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Species belonging to LAchnospira

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Lachnospira multiparus

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G- curved rods, anaerobe, mot +, tufts of flagella

Ferm acetic, propionic, lactic, and CO2

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Selenomonas

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Organism belonging to Selenomonas

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Selenomonas sputigena

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G-, anaerobic cocci, appear as diplo, mot-, oxi -, cat -

Family?

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Veillonellaceae

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Gums blue red color, swelling, bleeding, bone loss, tooth mobility

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Periodontal Disease

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Organisms in PEriodontal disease

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BActeroides melaninigenicus
- produces collagenase, gelatinase, and protease

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Clinical signs of an anaerobic infection

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Foul Putrid odor, location of infection near a mucosal surface, gas infected tissue, necrotic tissue, sulfur granules, actinmycosis,

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Lab Diagnosis of anaerobic infection

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tissue biopsy

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treatment of anaerobic infection

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surgical drainage and debridement
MEtronidazole

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Cellulose degraders

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Bacteroides, Ruminococcus, Butyrivibrio

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Starch degraders

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Bacteroides, Succinoomonas, Selenomonas

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Methane producer

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Methanobrevibacter ruminantium

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Anaerobic infections oxgeneous origin

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Clostridum difficle