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11 Cards in this Set
- Front
- Back
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Name the two types of solvent filters in HPLC |
1. Solvent Inlet Filter 2. Precolumn Filter |
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What is the composition and function of the solvent inlet filter? |
- Stainless steel or glass with 10 micron porosity. - Removes particulates from the solvent. |
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What is the composition and function of the precolumn filter? |
- Used between injector and guard column - 2-0.5 micron - Removes particulates from sample and autosample wear debris - Must be well designed to prevent dispersion. |
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What are the three basic functions of the solvent delivery system in HPLC? |
1. Provide accurate and constant flow 2. Provide accurate mobile phase compositions 3. Provide force necessary to push mobile phase through tightly packed column |
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Name the five performance parameters for HPLC pumps |
- Flow precision - Flow range - Delay volume - Pressure pulse - Composition precision |
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What are the two major designs of sample injectors? |
1. Automatic injectors 2. Manual injectors |
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What is required from a sample injector? |
Reproducible introduction of sample volume into the mobile phase flow |
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Name eight different types of HPLC detector |
1. UV-Vis 2. Mass Spectrometer 3. Refractive Index 4. Fluorescence 5. Light Scattering 6. Electrochemical 7. Radioactivity 8. Conductivity |
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Name seven detector performance characteristics |
1. Sensitivity (LoD, LoQ) 2. Selectivity 3. Linearity 4. Qualitative information 5. Reliability 6. Ease of use 7. Universality |
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What is the wavelength resolution of a diode array detector? |
Up to 1nm |
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What filter is used for wavelength calibration in a diode array detector? |
Holmium oxide filter |
