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8 Cards in this Set
- Front
- Back
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in exeriment 1 what are the contents of the tube that shows the color change?
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water, phosphate buffer, guiancol soln, potato, h2o2
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in experiment 1 what do you see happening in tube B? in the absense of a hydrogen donor, peroxidase can use h202 as a donor to form oxygen gas?
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tube b was cloudy and without quicol sultion to act as substrte it remained cloudy
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how did temperature affect the rate in experiment 2?
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tube a was cold and took latuer to change vs. room temp. tube c was heated and denatured the protein and did not change. the enzyme was more effective @ room temp
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in experiment 3 what happened to the enzyme?
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the enzyme had denatured and was cloudy in soltuion. it was unable to catalzye a reaction when placed with potato
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how are enzyme catalyze reactions dependent upon enzyme concentratoin?
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the higher the concentration of a substate with an enzyme present, the quicker the rxn goes s a very diluted concentration of a substrate
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baes on knowledge of structures of competitive and non competive inhibitors is Hg2+ like to be a competitive or non-competitive inhibitor, how do you know?
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non-competitve becaue a different size and shape vs. the binding to active site. rxn would stay the same and color change did occur
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is hydroxylamine likely to be a competive or non-competitive inhibitor. how do you know
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competitive, bece of binding to active sites and blocking them, no rxn and no color change
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if the enzyme in this experiment had been taken from a human, what do you think the optimum temp. whould have been why?
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the opt temperature for this enzyme could be at 37C bc it is the average body temp for a human being
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