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6 Cards in this Set
- Front
- Back
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Why do we use staining?
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Bacteria are transparent and difficult to see if doing a wet mount.
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Does Negative staining stain the backgroud or the organism?
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Negative staining will stain the background of the slide and not the organism
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What is the dye we use?
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dye used is Nigrosine (or India ink)
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What type of dye is this(acidic or basic)?
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- this is an acidic dye (chromophores have a negative charge), which
cannot penetrate the organism whose surface is negatively charged |
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What is Negative staining good for?
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useful for determining cell morphology and size
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What is the advantage to not heatfixing the bacteria?
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no heat-fixing means no cell distortion/shrinkage
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