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81 Cards in this Set

  • Front
  • Back
What would one use genetic engineering?
Change genes for practical purposes. Producing crops, livestock, insulin, etc...
Recombinant DNA
Combining DNA of two organisms.
What is a plasmid?
DNA
What is a vector?
A carrier for a gene to be cloned
What is the purpose of a restriction enzyme?
To cut the DNA
-protects from foreign DNA
Steps in cloning a gene
1.Get plasmid DNA
2. Get DNA with the gene you want
3. Cut plasmid DNA w/ restriction enzyme
4. mix plasmid & DNA
5. Makes recombinant DNA
6. recombinant DNa is taken up by a bacterial cell
7. Bacterial cell reproduces to form a clone of cells
Nucleic acid probes
stick to the gene you want by base pairing (A-T, C-G)
ex. DNA chips
In what organism are most genetically created medications made?
Bacteria
* What is DNA fingerprinting? How does it work and why is it used?
Looking at pieces of someone's DNA to compare individuals
*What is cloning and how is it done?
-Making an exact copy of an organism
-Take the DNA of who they want to clone & put it into a FERTILIZED EGG (take out existing DNA)
-Let it reproduce
-Will grow up to be exactly the same as the animal that was cloned, but will likely die younger.
If a normal sheep has 40 chromosomes, how many came from each parent?
20
How many genetic parents does a cloned animal have?
One
Could you use the DNA in an egg or sperm to make a clone?
No, because it's only 1/2 of your DNA
Evolution
- Change over time.
* What is natural selection?
Scientific theory for HOW evolution happened
*Which one is a theory?
Natural selection (A LOT of evidence)
*What are the forms of evidence to show that evolution has happened?
Fossils, homologous/similar structures, embryology, genetic similarities
(FHEG)
Fossils
-used to compare the past and today to see changes / similarities.
Ex. Whale evolution
*How do scientists believe that whales evolved?
-Lost their legs, nasal drift, hearing aids
(probably a mutation)
*What is a homologous structure?
-Body parts that are similar in different animals.
ex. Humans and monkeys
*What is an analogous structure?
Species who are not related but have similar features.
-serve similar functions but do not have the same structure.
Ex. Bats, flies, birds.
*Which is evidence for evolution and relationships between organisms?
Homologous structure or analogous?
Homologous structures
Embryology
Looking at embryos of different animals to see how similar & different they are.
Genetic similarities
-Comparing DNA & RNA sequences to find similarities & differences.
*How is DNA similar in all organisms?
ALL LIVING THINGS share the same set of nucleotides: ATCG in DNA, AUCG in RNA
- same set of codons for the same amino acids
What can we look at to find the difference in DNA?
-SEQUENCE of nucleotides
*How similar is our DNA to each other (human to human)?
99.9%
*How similar is human DNA to that of a chimpanzee?
Humans & chimps are 98% of the same DNA.
Which animal is most closely related to humans? Which animal is least closely related to humans?
Gorilla.
Frog.
The closer an organism is in classification to another organism...
the closer they are related
- means they share a common ancestor in the not so distant past
Three categories of Phylogenetic Tree of Life
Bacteria, Archaea, Eucarya
(Humans in Eucarya)
Is evolution a theory?
No
Is natural selection a theory?
Yes
How is a theory in science different from a theory in everyday life?
A scientific theory can be supported by repeated evidence.
*Four "shuns" of Natural Selection:
-Genetic Variation
-Overproduction of offspring
-Competition
-Differential survival + reproduction
Genetic variation
Natural variations or differences in populations
-inherited through DNA b/c of MEIOSIS & sexual reproduction
-variations occur occasionally because of mutations
Overproduction of offspring
Most individuals can make more babies than can survive in their habitat.
Competition
Too many animals being born than can survive causes competition.
-Compete over: space, food, water, shelter, mates, etc.
Differential survival & reproduction
Whomever is best matched with their environment will survive longer, those who survive longer can reproduce.
-passes their DNA to the next gen. so the best traits are those that get passed on.
-Good traits/genes increase in frequency
How many kingdoms of life are there?
6
Kingdoms of Life
Platae
Aninmalia
Fungi
Protista
Archaebacteria
Eubacteria
*Naming organisms
Domain
Kingdom
Phylum
Class
Order
Family
Genus
Species
*Three domains
Eukarya
Eubacteria
Archaebacteria
Eukarya
Kingdoms
_________
Protista
Fungi
Animalia
Plantae
Which domains contain bacteria?
Archaebacteria & Eucbacteria
Which domain contains animals?
Eukarya
Species
Group of natural populations that can breed.
(Used to say any organism that can breed within a species, but now we know wolves&dogs can breed = hybrids)
Which is the largest grouping of LIVING THINGS?
Domain
Which is the smallest, most specific grouping of living things?
Species
Prokaryotes:
do not have a nucleus
Three things about Eubacteria and Archabacteria
1. All are SINGLE CELLED PROKARYOTES.
2. Almost all have CELL WALLS.
3. Reproduce by FISSION (like mitosis or cloning).
*Domain Eubacteria
-Cell wall = peptidoglycan.
-Genes have NO introns.
-Proteins made differently.
*Domain Archaebacteria
-Most similar to us *
- Have a cell wall with NO peptidoglycan.

-extreme and OLD bacteria
- Genes HAVE introns
- Proteins are made like in eukaryotes
- DNA wrapped around proteins like us.
Which live in harsh environments like salt ponds and cow guts?
Archaebacteria
To which organisms are we most closely related?
Archaebacteria
Domain Eukarya
contains _ kingdoms
4.
Plantae, Protista, Animalia, Fungi
Domain Eukarya
-Genes HAVE introns
-Some are MULTICELLULAR, some unilinear.
-Some have cell walls, some don't.
-All are eukaryotes (DNA in nucleus).
Domain Eukarya
KINGDOM PROTISTA.
The "trash can" kingdom."
Single and multicellular
Genes HAVE introns
Some are photosynthetic, some aren't.
ex. Algae
Are algae plants?
No.
Domain Eukarya
Kingdom Fungi
-Single & multicellular
*Cells have cell walls of Chitin
-Genes HAVE introns.
-Hyphae dissolve food externally & bring it in.
Domain Eukarya
Kingdom Plantae
-Genes HAVE introns.
-Multicellular, plant cells.
-Photosynthetic: make own food.
-Cell walls of cellulose
Shared characteristics of animals?
Reproduce
Eat
Animal cells
Domain Eukarya
Kingdom Animalia
Multicellular
Made of ANIMAL cells
Heterotrophs (eat food).
Genes HAVE introns.
Use oxygen to make ATP (energy).
Phyla in Kingdom Animalia
38 animals iin phyla, but most species are found in the TOP 9 PHYLA.
Which animal phylum contains sponges?
Porifera
Platyhelminthes = Flatworms.
*First to have organ system
ex. tape worms, can do asexual reproduction
Annelida
-Bodies are segmented (divided into sections).
-*First phylum with a developed digestive system-mouth, "stomach"
Which animal phylum is the first to have a complete digestive system?
Annelida
Do mollusks have shells?
No
Arthropoda=crustaceans + insects
*Largest animal phylum, over 80% of all animals on planet are arthopods, over 1 million species.
Which is the largest animal phylum?
Arthropoda
Chordata (vetebrates) = animals with backbones
99% of animals with backbones
Which phylum contains reptiles?
Chordata
What is true about all chordrichthyes?
The breathe through gills
Mammals
*All produce milk for their young.
-breathe through lungs, have hair, almost all have live birth
Orders in Class Mammalia
Order Rodentia (rodents)
Order Carnivora (bears,lions,wolves)
Order Cetacea (whales & dolphins)
Order Primates (apes, gorillas,humans)
In what CLASS are humans?
Mammals
In what ORDER are humans?
Primates.
In what GENUS are humans?
Homo sapiens
EVERY LIVING THING HAS INTRONS...
EXCEPT EUBACTERIA
Two phlya (divisions) for Plants
Angiosperm & gynosperm