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19 Cards in this Set
- Front
- Back
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What are the three types of optical components in a filter cube for in Epi-fluorescence microscopy?
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1) Barrier Filter. (BA Filter)
2) Excitation Filter. (EX Filter) 3) Dichroic Mirror. (DM) |
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What is the abbreviation for a Barrier Filter in Epi-fluorescence microscopy?
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BA Filter
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What is the abbreviation for a Excitation Filter in Epi-fluorescence microscopy?
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EX Filter
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What is the abbreviation for a Dichroic Mirror in Epi-fluorescence microscopy?
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DM
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What does the excitation filter do in Epi-fluorescence microscopy?
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The excitation filter allows for light of specific wavelengths to pass through the filter while blocking light of all other wavelengths. This range of wavelengths that is ALLOWED to pass THROUGH the filter is referred to as the bandwith.
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What is an excitation filter's bandwith in Epi-fluorescence microscopy?
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This range of wavelengths that is ALLOWED to pass THROUGH the filter is referred to as the bandwith.
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What are the two types of barrier filters in Epi-fluorescence microscopy?
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1) Longpass filters (LP Filters)
2) Bandpass filters (BP Filters) |
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The abbreviation LP is short for what type of barrier filter in Epi-fluorescence microscopy?
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Longpass filter.
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The abbreviation BP is short for what type of barrier filterin Epi-fluorescence microscopy?
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Bandpass filter.
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What does the barrier filter do in in Epi-fluorescence microscopy?
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The barrier filter allows ONLY fluorescent light emitted by the specimen to pass blocking excitation light. This allows the specimen to be viewed without excess illumination (dark background)
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What does a long pass (LP) filter do in Epi-fluorescence microscopy?
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Long Pass filters block all light below a certain wavelength and allow all light above a certain wavelength to pass. (The Border Wavelength is called the cut-on wavelength)
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What is the cut-on wavelength in Epi-fluorescence microscopy?
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The cut-on wavelength is the border wavelength of a long pass filter between the light that is allowed to pass and the light that is not allowed to pass.
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What does a bandpass filter (BP) do in Epi-fluorescence microscopy?
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A bandpass filter allows light of a certain waveband to pass, but blocks all other light. (A specific range)
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What is a fluorophore in Epi-fluorescence microscopy?
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A fluorophore is a fluorescent chemical compound that can give off light upon light excitation.
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What is the range of the visible color spectrum?
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Typically 390 nanometers to 750 nanometers.
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What is a nanometer?
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One Billionth, with a B, of a meter.
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What is the abbreviation for a nanometer?
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nm
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What is the range of ultra violet rays (UV rays) in the spectrum?
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10nm to roughly 400nm
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What is "wavelength"?
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The distance between successive crests of a wave
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