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12 Cards in this Set
- Front
- Back
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What is the application for the EMB test?
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Used for isolation of fecal coliforms.
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What is in the EMB media?
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Peptone. Lactose. Sucrose. and the dyes eosin Y and methylene blue.
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What do the sugars for in the EMB test?
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Provide fermentable substrates to encourage growth of fecal coliforms.
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What are the dyes for in the EMB test?
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Inhibit the growth of Gram-positive organisms and under acidic conditions produce a dark purple complex accompanied by a green metallic sheen.
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What does the green metallic sheen indicate in an EMB test?
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Vigorous lactose or sucrose fermentation typical of fecal coliforms.
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What is the interpretation and Presumptive ID for a result of poor growth or no growth?
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Organism is inhibited by eosin and methylene blue. Gram-positive.
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What is the interpretation and Presumptive ID for a result of good growth?
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organism is not inhibited by eosin and methylene blue. Gram-negative.
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What is the interpretation and Presumptive ID for a result of pink and mucoid growth?
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Organism ferments lactose with little acid production. Possible coliform.
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What is the interpretation and Presumptive ID for a result of purple to black growth?
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Organism ferments lactose and/or sucrose with much acid production. Probable coliform.
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What is the interpretation and Presumptive ID for a result of colorless growth?
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organism does not ferment lactose or sucrose. No reaction. Noncoliform.
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What organism produces a metallic green sheen and why?
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Gram-positve organisms. Due to Dyes eosin Y and Methylene blue under acidic conditions.
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What organism produces a pink sheen and why?
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Gram-negative possible Coliform organisms due to fermented lactose and little acid production.
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