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9 Cards in this Set
- Front
- Back
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What is the Michaelis-Menton Equation?
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v = (Vmax*[S])/([S]+Km)
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What happens if the substrate (S) becomes very large?
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the velocity (v) becomes essentially Vmax
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What is the law of mass action? To what kinds of reactions does it apply?
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v = k[A][B], elementary
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Reaction rates are dependent on ______ and not on ______.
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a. concentration
b. amount |
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What is assumption #2 of the Michaelis-Menton equation (steady state assumption)?
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That [ES] stays relatively constant through most of the reaction and that the change thereof small
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What is assumption #1 of the Michaelis-Menton equation?
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i. [S]=S added. [S] never changes
ii. Therefore no P is made |
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What happens if you increase the catalytic rate constant (k2) or the total enzyme added ?
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The observed rate (vo) increases in direct proportion (i.e. double one, double the other)
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When [S] is much less than Km, what happens?
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The equation reduces to vo = Vmax[S]/ Km, and is linearly proportional to [S]
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What happens when [S] is much greater than Km?
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The equation reduces to vo = Vmax, and is independent of [S]
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