Embedding

Front 1

What is the purpose of embedding?

Back 1

Enclose the tissue with infiltration medium

Front 2

Types of embedding medium

Back 2

Paraffin

Water soluble waxes

Celloidin

Plastics

Front 3

Paraffin is the ___________

Back 3

Most common

Front 4

Water soluble waxes include:

Back 4

Carbowax

Agar

Gelatin

Front 5

Plastics medium include:

Back 5

Epoxy

Glycol methacrylate

Front 6

The paraffin melting point is commonly...

Back 6

55-58° C

Front 7

Paraffin should be kept at a temperature of about...

Back 7

2-4°C above MP

Front 8

Higher melting point affects:

Back 8

Increase in hardness

Increase in support

Easier to cut thin sections

Front 9

Lower melting point causes:

Back 9

Softer

Decrease support

Easier to ribbon

Front 10

List the main part of the embedding center

Back 10

Paraffin dispenser

Cold plate

Paraffin reservoir

Heated forceps

Warm storage

Mini chill plate

Front 11

Steps to embedding: (8)

Back 11

1) open cassette - 1 at a time

2) select mold size

3) fill mold with paraffin

4) orient specimen

5) embed

6) cover with cassette &fill

7) cool quickly

8) trim off paraffin

Front 12

Specimen orientation is the...

Back 12

Most important step

Front 13

Embed Elongated tissue

Back 13

Diagonally

Front 14

Embed tubular tissue

Back 14

Cross sectioned

Front 15

Embed epithelial demonstration

Back 15

On edge

Front 16

Embed multiple specimens

Back 16

In a line

Front 17

Embed bone

Back 17

Diagonally

Front 18

Skin should be embedded facing

Back 18

The same way/direction

Front 19

T/F: to avoid crystallizing it is important to slowly cool the block after orienting the specimen.

Back 19

False: needs to be cooled quickly

Front 20

Soft mushy tissue is from:

How to fix it:

Back 20

Inadequate processing

“Back up” tissue

Front 21

What is the “back up” process

Back 21

1) clearing with xylene

2) dehydrate with alcohol

3) fix

4 reprocess

Front 22

Prevent tissue carryover by

Back 22

Cleaning forceps between specimen

Open one cassette at a time

Front 23

How to ensure tissue is on the same level:

Back 23

Press tissue down uniformly and firmly

Work fast with small pieces

Let paraffin chill so tissue adheres to base

Front 24

How to avoid missing small stuff:

Back 24

Record # of pieces

Check lid of cassette

Carefully open lens paper

Front 25

How to ensure correct orientation:

Back 25

Mark tissue side facing up

Use embedding instructions

Front 26

Good practices include:

Back 26

Center tissue

Don’t overcrowd in mold

Cool blocks rapidly

Front 27

Which embedding medium is using mostly for large CNS cases in research ?

Back 27

Celloidin

Front 28

Putting tissue in a mold that is too small will:

Back 28

Give too little support

Front 29

Pressure is applied uniformly to the entire specimen during orientation to ensure that the specimen will:

Back 29

Be embedded flat

Front 30

List three quality control methods or good practices of embedding

Back 30

1)

2)

3)