Ecvp Miscellanous Questions Of The Day

Front 1

. Useful markers to differentiate Sertoli cells and germ cells? Give the 2 markers and the corresponding cells they stain.

Back 1

Sertoli cells : NSE +; germ cells: vimentin +

Hint 1

Question of the day, Nadine.

Front 2

Give for each of the following inclusions the location in the cell, the type of cell(s) in which they occur, and the stuff they consist of (indicating virus type if viral inclusion body):
Heinz body
Barr body
Guarnieri body
Bollinger b.
Civatte b.
Councilman b.
Mallory b.
Negri b.
Russel b.
Weibel-Palade b.
Joest-Degen b.

Back 2

Heinz body : intracytopl (no nucleus!), erys, denatured globin chains
Barr body : INIB (intranuclear), neutros, inactivated X chromosome in female animals.
Guarnieri body : eos. ICIB (intracytoplasmic), keratinocytes, Orthopoxviruses
Bollinger b. : eos. ICIB, keratinocytes, Ovipoxviruses
Civatte b. = apoptotic basal epidermal cells
Councilman b. = apoptotic hepatocytes
Mallory b. ICIB, hepatocytes, keratin intermediate filaments
Negri b. eos. ICIB, neurons, rabies virus
Russel b. : eos. ICIB in plasma cells (called Mott cells!), antibodies in vesicles/dilated ER (meningen verschillen)
Weibel-Palade b. (alleen electronenmicroscopie!!!) endothelial cells, P-selectin Voor Genpath!!!
Joest-Degen b. : INIB, CNS neurons (esp. hippocampus), Borna disease

Hint 2

Question of the day, Nadine.

Front 3

2 dog breeds suffering from severe combined immunodeficiency?

Back 3

Basset hound, Jack Russel terrier, Welsh corgi. (niet Irish Setter: canine leucocyte adhesion deficiency!!)

Hint 3

Question of the day, Nadine.

Front 4

Infectious cause(s) for thymic atrophy and/or thymitis in:
a. dog (2)
b. cat (2)
c. swine (1)
d. calve (1)
e. foal (1)
(Jubb 3, p.267 and foll.)

Back 4

a. dog (2) canine distemper, salmon poisoning disease (denk ook parvo maar staat er niet bij op die pagina...)
b. cat (2) feline panleukopenia virus, FIV
c. swine (1) (chronic) classical swine fever
d. calve (1) epizootic bovine abortion, bov. leukemia virus, bov. immunodeficiency virus
e. foal (1) equine herpesvirus type 1 abortion

Hint 4

Question of the day, Nadine.

Front 5

List 4 possible causes of thyroid hyperplasia

Back 5

5.
a. age/idiopathic (esp. cats)
b. alimentary insufficient supply of iode
c. in foetus: hypothyroidism of the mother
d. defects in metabolization of iode to active T3/T4 metabolites
e. dietary iode excess

Hint 5

Question of the day, Nadine.

Front 6

1.Define a ranula.

2. How is distinguished from a sialocele/salivary mucocele ?

Back 6

Ranula = cystic saliva-filled distention of the duct of the sublingual or submaxilary salivary gland, alongside the tongue
Salivary mucocele = pseudocyst NOT lined by epithelium but filled with saliva. Ranula is epithelial lined.

Hint 6

Question of the day, Nadine.

Front 7

synonym voor lethal white foal syndrome (geeft histo weer)
bij wat voor soort veulens (ras/kleur)?
oorzakelijke mutatie?

Back 7

aka colonic agangliosis.
American Paint Horses with Overo markings.
Homozygous dominant, mutations in endothelin-B receptor gene.

Hint 7

Question of the day, Nadine.

Front 8

1. Name 4 causes of multifocal necrotizing lymphadenitis, splenitis and
hepatitis in lab animals

Back 8

Tyzzer's, Y. pseudotuberculosis, Salmonellosis, Yersinia pestis inf.

Hint 8

Question of the day, Nadine

Front 9

4 DDx for fibrinous serositis in pigs?

Back 9

H. parasuis, Mycopl. hyorhinis, Sc. suis type II, septicemic
salmonellosis

Hint 9

Question of the day, Nadine

Front 10

Which of the following substances/agents can cause discoloration of
teeth? Tetracyclins, porphyrins, fluoride, canine distemper virus,
homogentisic acid (ochronosis).

Back 10

Tetracyclins, porphyrins, fluoride

Hint 10

Question of the day, Nadine

Front 11

List the 4 main histological features of portal vein hypoperfusion and
give 4 possible causes.

Back 11

a. Smaller or absent portal vein profiles in portal areas, proliferation
of hepatic arterioles, biliary ductular hyperplasia, portal fibrosis. b.
Congenital portosystemic shunts, arterioportal fistulas/anastomosis, primary
hypoplasia of the portal vein, portal vein obstruction.

Hint 11

Question of the day, Nadine

Front 12

List the 3 classical extrahepatic macroscopical lesions associated with
portal hypertension.

Back 12

Congestive splenomegaly, ascites, multiple acquired portosystemic
vessels.

Hint 12

Question of the day, Nadine

Front 13

Main 4 features of vena caval syndrome and cause?

Back 13

Dirofilaria immitis. Large numbers of worms in vena cava, acute hepatic
failure, DIC, intravascular hemolysis.

Hint 13

Question of the day, Nadine

Front 14

Mycoplasma diseases. Cause and host?
- Contagious bovine pleuropneumonia
- Contagious caprin pleuropneumonia
- Contagious agalactia
- Contagious agalactia/pleuropneum
- Calf pneumonia
- Enzootic pneumonia
- chronic respiratory disease
- infectious synovitis

Back 14

- M.m.mycoides SC : cattle
- M.m.m capripneumoniae: goat
- M. agalactiae: sheep, goats
- M.m.mycoides LC: goats
- M. bovis: cattle
- M. hyopneumoniae: pigs
- M. gallisepticum: poultry
- M. synoviae: poultry

Hint 14

CP 140, 85-96 (2009)

Front 15

Pathological findings in dogs with fatal heatstroke.
1. Most prevalent lesions (3)?
2. organs affected?

Back 15

1. hemorrhagic diathesis, microthrombosis and coagulative necrosis.
2. Diffuse edema: lung, brain, skin.
Congestion: spleen and liver.
Necrosis: mucosa of SI and LI, renal tubular epithelium, hepatic parenchyma and brain.

Hint 15

CP 140, 97-104 (2009)

Front 16

Regarding Oct4 in canine neoplasms: choose the correct statement(s):
Oct4 is:
- a member of the POU (pit, oct, unc) family of transcription factors.
- a tumor suppressor protein
- expressed during embryogenesis
- expressed in fewer than 25% of canine neoplasms studied by IHC
- in some tumors expressed in more than 90% of the neoplastic cells
- not expressed in normal nonneoplastic cells of adult canine tissue.

Back 16

Ok, -, ok, -, ok, ok

Hint 16

Vet pathol 44, 893-900 (2007)
studyquestion Sjoerd

Front 17

Canine distemper virus and signaling lymphocyte activation molecule (CD150).
1. IHC control dogs?
2. IHC acute disease CDV?

Back 17

1. + in lymphocytes and macrophages.
2. +, upregulated in many target organs: stomach, intestine, urinary bladder, lung, mesenteric lymph node.
Not in keratinocytes footpad and brain, that are + for CDV (unidentified receptor).

Hint 17

Vet pathol 44, 943-948 (2007)

Front 18

PCV-2 associated disease.
1. Other familymembers Circoviridae:
genus Circovirus (9)?
genus Gyrovirus (1)?
2. PCV-2 disease manifestations (6)?

Back 18

1. Genus circoviridae:
beak and feather disease virus, canary circovirus, goose circovirus, pigeon circovirus, PCV1, PCV2, tentatively duck circovirus, finch circovirus, gull circovirus
Genus Gyrovirus: chicken anemia virus (CAV).
2. Subclinical, systemic, respiratory, enteric, porcine dermatitis and nephropathy syndrome, reproductive failure.

Hint 18

JVDI 19, 519-615 (2007)

Front 19

PCV-2 associated diseases.
1. characteristics systemic infection?
2. characteristics respiratory disease?
3. characteristics enteric disease?

Back 19

1. weigthloss, paleness, white streaks kidney, mottled noncollapsed lungs.
- lymphohistiocytic to granulomatous lesions in lymphoid tissue and/or lung, liver, kidney, heart and intestines.
2. lymphohistiocytic to granulomatous interstitial pneumonia, peribronchiolar fibroplasia and mild-to-severe necrotizing and ulcerative bronchiolitis. may resemble influenza or porcine respiratory coronavirus.
3. increasingly common, 8-16 week old pigs.
- often clinically and grossly resemble subacute or chronic ileitis ass. with Lawsonia intracellularis, mucosa grossly thickened, enlarged mes. lnn.
Histo: granulomatous enteritis with abundant PCV2 IHC staining.

Hint 19

JVDI 19, 591-615 (2007)

Front 20

PCV-2 associated diseases.
4. characteristics reproductive disease?
5. characteristics PDNS?

Back 20

4. more abortions, stillbirths, fetal mummification and increased preweaning mortalities. Hallmark: nonsuppurative to necrotizing or fibrosing myocarditis with abundant PCV2 antigen in stillborn and neonatal pigs.
5. raised purple skin lesions progressingng to raised red-purple scabs with black centers, enlarged tan, waxy-appearing kidneys with petechial hemorrhages.
Hallmark histo: generalized vasculitis and glomerulonephritis, suggestive of type 3 hypersensitivity, characterized by antigen-antibody aggregates at certain tissue sites.

Hint 20

JVDI 19, 591-615 (2007)

Front 21

Porcine circovirus-2 in wasting and nonwasting pigs.
1. histo differences (5)?
2. PCV2 in healthy piglets?

Back 21

1. Wasting animals had more thymic atrophy, failure of normal pulmonary collapse, ascites, and less pronounced lymphoid germinal centers and proliferative paracortical T-dependent zones.
2. Yes PCV2a and -b both found in healthy pig lymph nodes.

Hint 21

JVDI 20, 274-282 (2008)

Front 22

PCV-2 in milk of lactating sows.
1. Detected in milk?
2. Detected in mammary glands?

Back 22

1. Yes, from day 1 in infected sows.
2. Yes, IHC +

Hint 22

CP 140, 208-211 (2009)

Front 23

PCV-2 associated cerebellar vasculitis in pigs with postweaning multisystemic wasting syndrome.
1. Clinic?
2. histo?
3. ICH and ISH?

Back 23

1. wasting and neurologic deficits, 6 weeks to 2 months of age.
2. acute hemorrhages and edema of cerebellar meninges and parenchyma due to a necrotizing vasculitis resulted in degeneration and necrosis of the gray and white matter.
3. + in endothelial cells in affected areas.

Hint 23

Vet pathol 44, 621-634 (2007)

Front 24

PCV-2 and PMWS in pigs.
1. Most common lesions in PMWS?
2. Inclusion bodies?

Back 24

1. Granulomatous lymphadenitis with lymphoid depletion.
Lymphoplasmacytic and histiocytic interstitial pneumonia.
Lymphoplasmacytic and histiocytic interstitial nephritis.
2. ICIB: amphophilic botrioid inclusion bodies in macrophages, bronchial, bronchial glandular and renal tubular epithelium. EM: also paracrystalline virus particles in nucleus. (pcv is dna-virus, so mechanism formation of ICIB is unknown)

Hint 24

Vet pathol 45, 640-644 (2008)

Front 25

Reproduction of PMWS in PCV-2 infected pigs.
1. Extra factors for intrauterine PCV-2 infected pigs to develop PMWS?

Back 25

1. infection with porcine parvovirus or immune stimulation.

Hint 25

Vet pathol 45, 842-848 (2008)