Ecvp Hematopoietic System 2007-2009

Front 1

Bone marrow pathology in dogs and cats with non-regenerative immune-mediated hemolytic anemia and pure red cell aplasia.
1. characterization of both diseases?
2. associated bone marrow changes, with which form most obvious?

Back 1

1. PRCA is form of IMHA : erythroid aplasia in the bone marrow.
- non-regenerative IMHA in two forms: with bone marrow erythroid hyperplasia or erythroid maturation arrest.
2. dysmyelopoiesis, myelonecrosis, myelofibrosis, interstitial edema, hemorrhage, acute inflammation and hemophagocytic syndrome, ass. with erythroid hyperplasia.

Hint 1

CP 138, 46-53 (2008)

Front 2

Multiple myeloma 1/interferon regulatory factor 4 (MUM1/IRF-4) in canine plasmacytoma.
1. % positive for MUM1/IRF-4, CD79a, CD20?
2. other tumors positive or negative?
3. sensitivity of MUM1/IRF-4?
4. cuteneous plasmacytomas in other species?
5. multiple myeloma in other species?

Back 2

1. 93% MUM1/IRF-4, 56% CD79a, 19,4% CD20, 45% both MUM and CD79, - CD20, 30% MUM and - CD79/CD20
2. some B lymphomas positive, other leukocytic or melanocytic tumors -.
3. MUM1: 93,5%, with CD79 95,5%.
4. cat and horse
5. cat, horse, cattle, pigs middle aged to aged.

Hint 2

Vet pathol 44, 875-884 (2007)

Front 3

Leishmania infantum in dogs.
1. correlation skin lesions with parasite load?
2. correlation of skin lesions with parasites in regional lymph nodes?

Back 3

1. skin without gross lesions have chronic inflammation, with less parasites in parotid lymph nodes than dogs with gross skin lesions.
2. parotid and cervical node were correlated with parasites in skin of nose and pinna.
Parasite load in mandibular lymph nodes was correlated to skin of external nose.

Hint 3

Vet pathol. 613-616 (2008)

Front 4

Feline myeloma-related disorders.
1. Survival rate and differentiation?
2. extramedullary involvement?
3. Agreement cytology/histology?
4. Sort of cells characterized? (5)

Back 4

1. Good differentiation had higher mean survival (254 vs 14 days).
2. in well differentiated tumors more often in cats than in humans (90% vs 20%)
3. good agreement
4. mature myeloma cell, proplasmacyte, plasmablast, giant myeloma cell, lymphoma cell.

Hint 4

vet pathol 45, 159-173 (2008)

Front 5

Postweaning multisystemic wasting syndrome in pigs.
1. Ultrastructural changes in histiocytes?

Back 5

1. - severe swelling and proliferation of mitochondria.
- proliferation and dilation of rough endoplasmatic reticulum and Golgi complex.
- small double membrane bound intracytoplasmic inclusions with viral-like particles 8-17 nm, paracrystalline array.
- large non/ or double membrane bound ICI with VLP in paracrystalline array.
-Intranuclear inclusion bodies, nonmembrane bound, virion of 10-13 nm.
- apoptotic lymphocytes (sometimes phagocytosed).

Hint 5

Vet pathol 46, 729-735 (2009)

Front 6

Epithelial cells in mediastinal lymph nodes of cattle.
1. association with carcinoma?
2. IHC characteristics? Vimentin, factor 8 related antigen, CD68, cytokeratin?

Back 6

1. Can be, but in almost 60% of lymph nodes epithelioid cells were observed.
2. Vimentin, factor 8, CD68 -, cytokeratin +: identical to bovine mesothelial cells.

Hint 6

Vet pathol 46, 430-438 (2009)

Front 7

Mycobacterium bovis granulomas in retropharyngeal lymph node of cattle.
1. Chronology of changes in iNOS?
2. in CD4+, CD8+, CD68+, gamma/delta Tcells?
3. CD68 stains what cells?

Back 7

1. iNOS = inducible nitric oxide synthase, day 15 to 60, diminished from day 90.
2. CD4 and CD68 consistently high,
CD8 and gamma/delta diminished >60 days.
3. epithelioid macrophages.

Hint 7

Vet pathol 44, 863-874 (2007)

Front 8

Corynebacterium pseudotuberculosis and ovine caseous lymphadenitis.
1. Bacterial characteristics and family members?
2. Virulence factors?
3. Species affected and lesions?

Back 8

1. Gram+, nonmotile pleomorphic rod, family Actinomycetaceae (Mycobacterium, Rhodococcus, Nocardia), multiplies in macrophages, mechanism unknown.
Two strains: biovar ovis (biotype 1): sheep/goat
biovar equi (biotype 2): horse/cattle
2. Phospholipase D (PLD): exotoxin, desctruction of cell membranes.
- impairs ovine neutrophil chemotaxis
- increase vascular permeability
- activates complementary pathway→ depleting complement→ protection from opsonisation.
Mycolic acid: cytotoxic
- degeneration and death of phagocytosing leucocytes.
3. Sheep: severe chronic lymphadenitis, esp. superficial lymph nodes of torso, visceral and pulmonary lesions common
- Goat: superficial lymph nodes, head and neck.
- Horse: ulcerative lymphadenitis. Equine folliculitis and furunculosis.
deep seated subcutaneous abscessation, rare generilazed, abortion or mastitis.
Cattle: deep subcuteneous abscesse, ulcerative lymphangitis and rare mastitis
exotics: llama, alpaca, camel, deer, pig, coypus, primate, duck, hedgehog.

Hint 8

CP 137, 179-210 (2007)

Front 9

Corynebacterium pseudotuberculosis and ovine caseous lymphadenitis.
4. Transmission?
5. Histo?
6. Pathogenesis?
7. Diagnosis?
8. DDx (sporadic)?

Back 9

4. -environment and fomites, aerosols, direct contact, flies
5. first neutrophils, then macrophages. Central necrosis, minor mineralization, encapsulation, enlargement with capsular reformation (onion rings).
6. entry (wounds)→ local lymph nodes→ <24 hrs microsc. pyogranulomas→grow and coalesce→ further expansion via lymphatics or blood.
7. Culture. Serology based on PLD toxin only in some species.
8. Actinobacillus licheniformis, arcanobacterium pyogenes, staphylococcus aureus subsp. anaerobius.

Hint 9

CP 137, 179-210 (2007)

Front 10

Frequency and distribution of nerves in lymph nodes and Peyer's patches in sheep with and without scrapie.
1. Connection with number of nerves and scrapie infection?

Back 10

1. no.
No nerves found in regular lymph node germ centers, only occ. together with scrapie in Peyers patches.

Hint 10

Vet pathol 46, 233-240 (2009)