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35 Cards in this Set
- Front
- Back
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TSA
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tryptic soy agar
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Mac
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MacConkey agar
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EMB
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eosin methylene blue agar
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MSA
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mannitol salt agar
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used to isolate and differentiate entrobes based on their ability to ferment lactose
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MacConkey agar
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lactose
bile salts neutral red dye crystal violet dye |
MacConkey agar
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acid acumulation from lactose fermentation turns the dye red
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MacConkey agar
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lactose fermentors turn a shade of red
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MacConkey agar
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non lactose fermentors remain their original color
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MacConkey agar
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formulations without crystal violet allow a growth of enterococcus and staphylcoccus which ferment the lactose and appear pink on the medium
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MacConkey agar
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used for isolation of fecal coliforms
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eosin methylene blue agar
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peptone
lactose sucrose eosin Y dye methylene blue dye |
eosin methylene blue agar
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inhibits growth of gram positive organisms
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eosin methylene blue agar
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green metallic sheen indicates lactose or sucrose fermentation
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eosin methylene blue agar
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smaller amounts of acid will turn pink
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eosin methylene blue agar
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non fermentors remain their normal color or take on the color of the media
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eosin methylene blue agar
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used for isolation and differentiation of pathogenic staphylococci
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Mannitol salt agar
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carbohydrate mannitol
7.5% sodium chloride (NaCl) pH indicator phenol red dye |
Mannitol salt agar
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phenol red dye is yellow bellow pH 6.8
red from pH 7.4-8.4 pink above pH 8.4 |
Mannitol salt agar
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selective for staphylococci
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Mannitol salt agar
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pathogenic staff ferment mannitol which produces acid turning the pH indicator yellow
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Mannitol salt agar
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non pathogenic staph will grow but produce no color change
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Mannitol salt agar
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do not heat fix
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negative stains
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heat fix
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positive stains
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crystal violet (primary stain)
grams iodine (mordent) safranin (counter stain) |
gram stain
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carbolfuchsin
methylene blue |
acid fast stain
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malachite green
safranin |
endospore stain
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Mac
pH > 6.8 |
colorless
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Mac
pH > 6.8 |
red
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Mac
formulation of the agar without crystal violet allow growth of enterococcus and staphylococcus which ferment the lactose and appear |
pink
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EMB
indicates lactose or sucrose fermentation |
green metallic sheen
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EMB
smaller amounts of acid will turn |
pink
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MSA
pH < 6.8 |
yellow
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MSA
pH from 7.4 - 8.4 |
red
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MSA
pH > 8.4 |
pink
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