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37 Cards in this Set

  • Front
  • Back
Which of the following labelled molecules would be most useful in measuring the synthesis of DNA in intact living cells? (multiple choice)
Uridine
dTTP
Thymidine
dUMP
Thymidine
3.) This base is in the ______ conformation? (multiple choice)
Syn
Anti
Anti
5.) Which of the following is (are) not characteristic of the Watson and Crick DNA double helix? (multiple choice)
The two strands wrap around each other in a right handed helix

The phosphodiester backbone is located on the exterior surface of the helix

It is susceptible to alkaline hydrolysis
It absorbs light in the ultraviolet
The strength of the bonds between the two strands is a function of the GC to AT ratio

2 and 4
It is susceptible to alkaline hydrolysis
6.) Chargaff’s experiments on DNA base composition demonstrated that … (multiple choice)
The ratio of A to T is always close to one
The ratio of G to C is always close to one
G always base pairs with C
A always base pairs with T
All the above
1 and 2
1 and 4
2 and 3
1 and 2
1.) Okazaki fragments are synthesized as part of: (multiple choice)
The leading strand
The lagging strand
The lagging strand
2.) Why is a topoisomerase necessary for DNA replication? (multiple choice)
To separate strands to be copied
To relieve positive supercoiling ahead of the replication fork

To position the primase on the lagging strand
To allow single stranded DNA binding protein to protect the DNA
To relieve positive supercoiling ahead of the replication fork
3.) A DNA helicase is necessary for DNA replication because: (multiple choice)
The strands are antiparallel
The molecule has an alpha helical structure
The stands must be separated for replication
All of the above
1 and 2
2 and 3
2 and 3
4.) What enzymatic activity is the error checking mechanism of DNA polymerase? (multiple choice)
5’ to 3’ exonuclease
3’ to 5’ exonuclease
5’ to 3’ endonuclease
3’ to 5’ endonuclease
3’ to 5’ exonuclease
5.) Which of the following biosynthetic reactions are catalyzed by a processive enzyme? (multiple choice)
DNA replication
Glycogen synthesis
Fatty acid biosynthesis
RNA synthesis
All of the above
1 and 4
All of the above
6.) Which of the following biosynthetic reactions are catalyzed by a processive enzyme? (multiple choice)
DNA replication
Glycogen synthesis
Fatty acid biosynthesis
RNA synthesis
All of the above
1 and 4
All of the above
1.) The process of mismatch repair in E. coli depends upon … (multiple choice)

An unmethylated daughter strand following replication
Recognition of methylated GATC
Action of an endonuclease
Action of Pol I
1, 2 and 3
1 and 2
1, 2 and 3
6.) The process of mismatch repair in E. coli depends upon … (multiple choice)

An unmethylated daughter strand following replication
Recognition of methylated GATC
Action of an endonuclease
Action of Pol I
1, 2 and 3
1 and 2
Cytosine is occasionly deaminated
7.) Uracil N glycohydrolase is necessary because… (multiple choice)
2, 3 and 5
8.) The action of a glycosylase requires the subsequent action of an ______ to complete the process of repair. (multiple choice)
Is incorporated into DNA
Causes a mutation leading to histidine dependence for growth
Causes a mutation leading to loss of histidine dependence for growth

Is metabolized by the liver extract
Causes a mutation leading to loss of histidine dependence for growth
10.) The liver extract is sometimes used in the Ames test because: (multiple choice)


Liver is the tissue that metabolizes drugs
Sometimes chemicals can be metabolized and become mutagenic
Some chemicals can lose their mutagenicity following metabolism

All the above
1 and 2
All the above
11.) Which of the following represent transversion mutations? (multiple choice)

A to T
T to A
G to A
C to T
All the above
1 and 2
3 and 4
3 and 4
12.) Telomeres shorten after each round of replication because: (multiple choice)
The leading strand must loop out to be replicated
The lagging strand must loop out to be replicated
An individual DNA polymerase complex is unable to function as a monomeric complex

1 and 3
2 and 3
2 and 3
During DNA synthesis the activity of a topoisomerase is needed. It is needed because during the course of DNA synthesis:
b. unwinding the strands at the replication fork induces greater twisting of the two strands ahead of the replication fork. Topoisomerase action relaxes this.
In E. coli the function of dnaA is to:
c. bind to the sequences representing the origin of replication
If the deoxynucleotide triphosphates serving as substrates for DNA synthesis were radioactively labelled with phosphorus 32 (32P) the phosphate which should be labelled in order for the label to be incorporated into the DNA should be at the:
a. alpha position
In a relaxed DNA molecule the linkage number is equal to:
a. the number of complete turns of the double helix
The DNA structure with the largest difference between the major and minor grooves is:
b. B form
DNA synthesis in E. coli can be slowed by the addition of an inhibitor of the RNA polymerase. This led to the discovery that...
c. the primer used to initiate DNA synthesis is an RNA molecule.
The function of a DNA helicase is to:
c. unwind the DNA ahead of the replication fork
The writhing number of the DNA double helix is a function of the:
e. b and c
The A form of DNA is found most often when:
b. there is low humidity and a close to equivalent GC and AT content
A topoisomerase is an enzyme which
f. a and c
From the E. coli origin of replication DNA synthesis proceeds:
b. On both strands, in both directions simultaneously moving away from the origin of replication
DNA replication proceeds in the:
a. 5' to 3' direction
Which of the following characteristics of DNA polymerase is (are) most important for the complete replication of the entire genome?
f. a and b
In E. coli in the presence of an inhibitor of RNA polymerase
c. DNA synthesis would not occur
In E. coli the primer which is synthesized to provide a free 3' hydroxyl for addition of nucleotides is ultimately removed by the action of:
b. the 5' to 3' exonuclease activity of Pol I
Synthesis of Okazaki fragments is necessary because:
d. a and b
The proofreading activity of DNA polymerase is attributed to
b. the 3' to 5' exonuclease activity
Which of the following best describes the steps necessary for the initiation of DNA synthesis in prokaryotes?
g. a, c, d and e
E. coli DNA polymerase I has three different enzymatic activities. Which of these is a correct description of the different activities?
f. c, d and e
Which of the following activities is characteristic of the telomerase enzyme?
e. a and c