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15 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Nucleic Acid Hybridization |
Exploits the agility of a single stranded nucleic acid to form double stranded molecule by complementary hydrogen bar pairing |
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Hybridization Probe |
Known population of nucleic acid molecules or synthetic oligonucleotide |
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DNA Denaturation |
Breaking of DNA hydrogen bonding often by heating |
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Heteroduplex |
The resulting of a combination of a test sample DNA strand and a probe section which is useful in confirming the presence of a specific gene sequence |
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Hybridization Stingency |
Stringency is the specificity with which the bonding will occur. Can be controlled to study homologous traits. |
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Stringency Regulatory Factors |
- Temperature - Ion presence - Probe length |
3 factors |
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Labeled Probe |
1. Test sample is I mobilized 2. Labeled sample is introduced in and allowed to bind 3. Excess labels are removed |
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Labeled Sample |
1. Probe is immobilized 2. Test sample are added in and allowed to bind freely 3. Excess sample is washed out |
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Microarray |
Used large scale hybridization to immobilize probe into a high density grid Single probe is used to hybridized and non specific binding is washed out
Scanned to detect signal density |
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Two colour microarray |
Two RNA samples are converted to cDNA and labels with different fluorescent colours We’d into the hybridization system at the same time Microarray detects the signal to see if one or both colours are present |
Useful in comparative genomic hybridization |
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Sanger dideoxy sequencing |
Small levels of one of 4 fluorescently labeled ddNTPs are added into 4 separate reactions and thus will help sequence DNA |
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Next-Generation Sequencing |
Sequencing technique that allows for acquisition of DNA data while the method is being run rather than after its completion
May or may not require amplification by PCR |
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Iterative Pyrosequecing |
Sequencing that records the light energy released from cleavage of a phosphate bond resulting in a pyrophosphate formation |
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Enzymes in iterative pyrosequrncing |
1. DNA Polymerase 2. Sulfurylase 3. Luciferase |
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Iterative pyrosequencing technique |
Nucleotides are rotated one by one and cycled as the system recognizes any possible fluorescence
Apyrase is used to degrade any unincorporated dNTPS and excess ATP before next steps |
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